HPLC法测定复方氯霉素乳膏中主药的含量

目的:建立以高效液相色谱法测定复方氯霉素乳膏中氯霉素与氨苯砜含量的方法。方法:色谱柱为Hypersil ODS,流动相为含0.1%庚烷磺酸钠的溶液(取0.1%庚烷磺酸钠溶液500mL与二甲基甲酰胺5mL、冰醋酸0.5mL,混匀)-乙腈(75∶25),流速为1.0mL·min-1,检测波长为272nm,进样量为20μL。结果:氯霉素与氨苯砜检测浓度的线性范围分别为25.59~153.54、12.57~75.42μg·mL-1(r=0.9999);平均回收率分别为100.62%(RSD=0.92%)、100.93%(RSD=1.22%)。结论:本方法简便、准确,可用于该复方制剂的含量测定。

Determination of Chloramphenicol and Dapsone in Compound Chloramphenicol Cream by HPLC

OBJECTIVE： To establish the HPLC method for the determination of chloramphenicol and dapsone in compound chloramphenicol cream. METHODS： The analytical column was Hypersil ODS and the mobile phase consisted of 0.1% sodium heptanesulfonate solution （a mixture of 0.1% sodium heptanesulfonate 500 mL and dimethylformamide 5 mL plus acetic acid glacial 0.5 mL） acetonitrile （75 ： 25） at a flow rate of 1.0 mL·min^-1. The detection wavelength was 272 nm and the sample size was 20 μL. RESULTS： The linear ranges of chloramphenicol and dapsone were 25.59-153.54μg·mL^-1（r = 0.9999） and 12.57-75.42 μg·mL^-1（r = 0.9999）, respectively. The average recovery rates were 100.62% （RSD = 0.92% ） and 100.93% （RSD = 1.22% ）, respectively. CONCLUSION： The method is simple and accurate and can be used for the quality control of compound chloramphenicol cream.