| 非病毒载体法转染野生型P^53基因对肺癌细胞生长特性的影响 |
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| 引用本文： | 吴克,赖祥进,刘会生,全家妩. 非病毒载体法转染野生型P^53基因对肺癌细胞生长特性的影响[J]. 广西医科大学学报, 2000, 17(3): 399-403 |
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| 作者姓名： | 吴克 赖祥进 刘会生 全家妩 |
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| 作者单位： | 1. 卫生部武汉生物制品研究所武汉 430060
2. 广西医科大学生化教研室南宁 530 021 |
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| 摘 要： | 目的：研究非病毒载体法基因转染的最适条件及其潜在价值。方法：利用脂质体介导和配体定向法将带有野生型Ｐ＾５３基因（Ｗｔ－Ｐ＾５３）ｃＤＮＡ的质粒引入细胞，用直接镜检、软琼脂培养、四甲基偶氮唑（ＭＴＴ）法和乳酸脱氢酶法等研究细胞生长特性的改变情结果：绝大多数细胞被转染，肿瘤细胞生长受到明显抑制而发生凋亡。结论：本方法对于基因体外转染是可行的，并且有可能将来用于小细胞肺癌的Ｐ＾５３基因治疗。
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| 关 键 词： | 肺癌 基因转染 P53基因 非病毒载体法 |
| 修稿时间： | 1999-04-25 |
IMPACT STUDY ON LUNG CANCER CELL CULTURE AFTER WILD-TYPE P53 GENE TRANSFECTION WITH NON-VIRAL VECTOR |
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| Wu Ke,Lai Xiangjin,Liu Huisheng,et al. IMPACT STUDY ON LUNG CANCER CELL CULTURE AFTER WILD-TYPE P53 GENE TRANSFECTION WITH NON-VIRAL VECTOR[J]. Journal of Guangxi Medical University, 2000, 17(3): 399-403 |
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| Authors: | Wu Ke Lai Xiangjin Liu Huisheng et al |
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| Affiliation: | Wu Ke,Lai Xiangjin*,Liu Huisheng,et al |
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| Abstract: | Objective:To optimize the P 53 gene transfection conditions and value the nonviral gene vector methodMethods:The plasmids with wildtype P 53 gene (WtP 53 ) were liposomemediated,ligandtargeted,and introduced into cancer cells,then the comparative studies were made with direct microscopy,soft agar culture,monotetrazolium (MTT) analysis and lactate dehydrogenase (LDH) testResults:Most cells were transfected,the growth of which was apparently arrested while apoptosis was observedConclusion:The gene transfection protocol was practical in vitro and might be useful in P 53 gene therapy for small cell lung cancer |
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| Keywords: | P~(53) gene transfection lung cancer |
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