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| 加味三棱丸对子宫内膜异位症黏附分子ICAM-1，CD44表达的影响 | |
| 引用本文： | 李卓恒 李傲 陈刚 何瑶 王静 徐晓玉. 加味三棱丸对子宫内膜异位症黏附分子ICAM-1，CD44表达的影响[J]. 中国中药杂志, 2009, 34(10): 1285 |
| 作者姓名： | 李卓恒 李傲 陈刚 何瑶 王静 徐晓玉 |
| 作者单位： | 1. 重庆医科大学药学院,重庆,400016 2. 重庆工学院化学与生物工程学院,重庆,400050 3. 重庆工商大学药物研究中心,重庆,400067 4. 重庆医科大学药学院,重庆,400016;西南大学药学与中医药学院,重庆,400716 |
| 基金项目： | 重庆市科委应用基础研究项目［(2002)18号-99］ |
| 摘 要： | 目的:研究加味三棱丸(SLW)对子宫内膜异位症移植物的抑制作用和探讨对黏附分子ICAM-1,CD44表达的影响.方法:采用子宫内膜自体移植法建立大鼠子宫内膜异位症模型,用游标卡尺、HE染色和免疫组化分别检测子宫内膜异位症移植物体积变化、异位内膜组织形态学改变和黏附分子ICAM-1,CD44的表达.结果:SLW 0.11,1.02 g·kg~(-1)均可以明显减小子宫内膜异位症移植物的体积;显微形态显示:给药组与模型组比较其病理改变程度减轻,异位内膜为薄单层细胞排列而非腺体数目多,细胞呈多层排列,腺上皮呈扁平或矮立方形,未见假腺体改变;SLW 1.02,0.11 g·kg~(-1)均能明显降低了异位子宫内膜黏附分子ICAM-1,CD44的蛋白表达.结论:SLW可显著抑制子宫内膜异位症移植物生长,其作用机制可能与抑制黏附分子ICAM-1,CD44的蛋白表达水平有关. |
| 关 键 词： | 加味三棱丸 子宫内膜异位症 黏附分子 ICAM-1 CD44 |
Inhibitory effect of Sanlengwan on expression of adhesion molecules ICAM-1, CD44 in rats of endometriosis |
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| LI Zhuo-Heng-,Li-Ao-,Chen-Gang-,He-Yao-,Wang-Jing-,Xu-Xiao-Yu-. Inhibitory effect of Sanlengwan on expression of adhesion molecules ICAM-1, CD44 in rats of endometriosis[J]. China Journal of Chinese Materia Medica, 2009, 34(10): 1285 | |
| Authors: | LI Zhuo-Heng- Li-Ao- Chen-Gang- He-Yao- Wang-Jing- Xu-Xiao-Yu- |
| Affiliation: | 1. Pharmaceutical Sciences Collage of Chongqing University of Medical Science, Chongqing 400016, China; 2. Chemistry and Biotechnology Collage of Chongqing Institute of Technology, Chongqing 400050, China; 3. Research Center of Medical Chemistry and Chemical Biology, Chongqing Technology and Business University, Chongqing 400067, China; 4. Pharmaceutical Sciences and Chinese Medical Pharmaceutical Sciences Collage of Xinan University, Chongqing 400716, China |
| Abstract: | Objective: To explore the effect of Sanlengwan (SLW) on adhesion molecules expression and the accretion in ectopic endometrium of rats. Method: Endometriosis was established by surgical implant of endometrial tissue which belong to its body. Forty EMS model rats were randomly divided into five groups: model control group, three dose of SLW groups and anastrozole group. Meanwhile, eight normol rats were used as the normal group. All the rats were treated for 4 weeks respectively, and the volume of grafts were measured by vernier caliper, morphological changes were measured by HE stain, and the adhesion molecules, ICAM-1 and CD44 protein, were also measured by immunohistochemical test before and after treatment of SLW. Result: SLW markedly reduced the volume of grafts, improve the morphological characters and decreased the expression of ICAM-1 and CD44 in ectopic endometrial tissue. Conclusion: SLW can inhibit the accretion of ectopic endometrium tissue of rats, and its mechanism might be associated with inhibiting the expression of ICAM-1 and CD44 protein. |
| Keywords: | Sanlengwan (SLW) EMS adhesion molecule ICAM-1 CD44 |
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