胆汁制钩吻总碱抑制结肠癌细胞增殖的作用研究

目的 探讨胆汁制钩吻总碱（胆钩吻总碱）体外抗肿瘤效果，旨在为钩吻减毒后存效的研究提供科学依据。方法 通过CCK-8检测观察胆钩吻总碱对人结肠HCT-116细胞、人神经胶质瘤U87细胞、人肝癌HepG2细胞、人肺癌A549细胞的增殖作用，进一步以结肠癌HCT-116为研究对象，以不同浓度胆钩吻总碱（50、100、200 μg•mL^-1）干预HCT-116细胞，通过流式细胞技术检测其对细胞周期阻滞的影响；Annexin V FITC/PI流式细胞术检测HCT-116细胞凋亡情况；进一步从蛋白水平检测凋亡相关蛋白表达。结果 在钩吻总生物碱IC50浓度下，胆钩吻总碱对U87、A549、HepG2、HCT-116肿瘤细胞的增殖抑制率均高于钩吻总生物碱组，并且各组之间的差异具有统计学意义（P＜0.01）。与空白组相比，不同浓度的胆钩吻总碱处理（50、100、200 μg•mL^-1）可有效降低HCT-116细胞的集落形成，并将细胞周期阻滞在G2/M期。胆钩吻总碱还能引发结肠癌HCT-116细胞的晚期凋亡，并对凋亡相关蛋白Bax、Bcl-2、Caspase-3起到调控作用。结论 胆钩吻总碱可以通过调控结肠癌HCT-116细胞周期和凋亡相关蛋白的表达来抑制其增殖。

Research on the suppressive effects of alkaloids of bile-processed Gelsemium elegans on colon cancer cell proliferation

Objective To investigate the in vitro anti-tumor effect of the alkaloids of bile-processed Gelsemium elegans (ABPG) and provide scientific basis for the study of the post-detoxification efficacy of Gelsemium elegans(GE). Methods The proliferation effects of BPGA on human colon cancer HCT-116 cells, human glioblastoma U87 cells, human hepatocellular carcinoma HepG2 cells, and human lung adenocarcinoma A549 cells were assessed using CCK-8 assay. Subsequently, focusing on colon cancer HCT-116 cells, different concentrations of BPGA (50, 100, 200 μg•mL^-1) were used to intervene with the cells. The influence on cell cycle arrest was determined by flow cytometry. Cell apoptosis was detected using Annexin V FITC/PI flow cytometry assay. Furthermore, investigate apoptosis-related protein expression from a protein level. Results At the IC₅₀ concentration of GE, the proliferation inhibitory rate of ABPG on U87, A549, HepG2, and HCT-116 tumor cells was higher than that of the GE group, and the differences between the groups were statistically significant (P<0.01). Compared with the control group, treatment with different concentrations of BPGA (50, 100, 200 μg•mL^-1) effectively reduced colony formation of HCT-116 cells and arrested the cell cycle in the G2/M phase. BPGA also induced late-stage apoptosis in colon cancer HCT-116 cells and regulated apoptosis-related proteins Bax, Bcl-2, and Caspase-3. Conclusion BPGA can suppress the proliferation of colon cancer HCT-116 cells by regulating the expression of cell cycle and apoptosis-related proteins.