血清中的免疫炎症生物标志物与慢性湿疹病人的疾病严重程度相关性研究

目的探讨慢性湿疹病人血清免疫炎症生物标志物与疾病严重程度的相关性。方法将 2021年 9月至 2022年 8月于武汉市中医医院接受治疗的 100例慢性湿疹病人纳入研究，包括局限性湿疹 69例、泛发性湿疹 31例；根据疾病严重程度分为轻度组 37例、中度组 34例和重度组 29例；将同期该院 40例健康体检者纳入对照组。采用酶联免疫吸附测定检测血清免疫炎症生物标志物白细胞介素( IL)-2、IL-4、IL-5、IL-6、γ干扰素( IFN-γ)、 IL-10、IL-17、IL-18、IL-23、IL-33、肿瘤坏死因子 α(TNF-α)水平；采用流式细胞仪检测外周血分化抗原( CD)4+、CD8+水平；采用 Pearson相关分析慢性湿疹病人血清免疫炎症生物标志物与湿疹面积及严重度指数( EASI)评分的相关性。结果对照组、轻度组、中度组、重度组慢性湿疹病人血清 IL-2［(10.51±2.10) μg/L比( 12.94±2.26)μg/L比( 15.03±2.34)μg/L比( 17.94±2.56)μg/L］、 IL-4、IL-5、IL-6、IL-10、IL-17、IL-18、IL-23、IL-33、TNF-α、 IFN-γ、CD8+CD4+依次升高， ［( 43.24±6.79)%比( 37.04±4.25)%比( 29.12±2.78)%比( 25.62±2.35)%］依次降低( P<0.05)。局限性与泛发性慢性湿疹病人血清 IL-2、IL-4、IL-5、IL-6、IL-10、IL-17、IL-18、IL-23、IL-33、TNF-α、IFN-γ、CD4+、CD8+差异无统计学意义(P>0.05)。慢性湿疹病人 EASI评分与血清 IL-2、IL-4、IL-5、IL-6、IL-10、IL-17、IL-18、IL-23、IL-33、TNF-α、IFN-γ、CD8+呈正相关，与 CD4+呈负相关( P<0.05)。结论血清免疫炎症生物标志物与慢性湿疹病人病情严重程度存在显著相关性，可考虑将各指标作为病情评估的相关辅助指标，在临床中推广应用。

Study on the correlation between serum immune inflammatory biomarkers and disease severity in patients with chronic eczema

Objective To investigate the correlation between serum immune inflammatory biomarkers and disease severity in patients with chronic eczema.Methods One-hundred patients with chronic eczema who were treated in Wuhan Traditional Chinese MedicineHospital from September 2021 to August 2022 were included in the study, including 69 patients with localized eczema and 31 patientswith generalized eczema; according to the severity of the disease, the patients were divided into mild group (37 cases), moderate group(34 cases) and severe group (29 cases); at the same time, 40 healthy people were included in the control group. Enzyme-linked immuno sorbent assay (ELISA) was used to detect the levels of serum immune inflammatory biomarkers interleukin (IL)-2, IL-4, IL-5, IL-6, in terferon-gamma (IFN-γ), IL-10, IL-17, IL-18, IL-23, IL-33, and tumor necrosis factor-α (TNF-α); flow cytometry was used to detect the levels of CD4+ and CD8+ in peripheral blood; Pearson correlation was used to analyze the correlation between serum immune inflammatory biomarkers and eczema area and severity index (EASI) scores in patients with chronic eczema.Results Serum IL-2 [(10.51±2.10) μg/L vs. (12.94±2.26) μg/L vs. (15.03±2.34) μg/L vs. (17.94±2.56) μg/L], IL-4, IL-5, IL-6, IL-10, IL-17, IL-18, IL-23, IL-33, TNF-α, IFN-γ and CD8+ in patients with chronic eczema in the control group, mild group, moderate group and severe group increased in turn, and CD4+ [(43.24±6.79)% vs. (37.04±4.25)% vs. (29.12±2.78)% vs. (25.62±2.35)%] decreased in turn (P<0.05). There was no obvious difference in serum IL-2, IL-4, IL-5, IL-6, IL-10, IL-17, IL-18, IL-23, IL-33, TNF-α, IFN-γ, CD4+, and CD8+ between patients with lo calized and patients with generalized chronic eczema (P>0.05). The EASI score of chronic eczema patients was positively correlated with serum IL-2, IL-4, IL-5, IL-6, IL-10, IL-17, IL-18, IL-23, IL-33, TNF-α, IFN-γ, CD8+, and negatively correlated with CD4+ (P< 0.05).Conclusion There is a obvious correlation between serum immune inflammatory biomarkers and the severity of chronic eczema,and these indicators can be considered as relevant auxiliary indicators for disease assessment, which can be popularized and applied inclinical practice.