乳腺癌14-3-3 sigma基因表达及其临床意义

目的 探讨乳腺癌14—3—3sigma基因表达及其临床意义。方法 采用RT—PCR和western—blot方法，分别对40例乳腺癌患者肿瘤组织和18例乳腺良性病变的乳腺组织，半定量检测14—3—3sigma基因的表达情况。结果 40例乳腺癌患者中，有35例（35／40，87．5％）没有检测出14—3—3sigma mRNA；western—blot也证明，40例中有32例14—3—3sigma蛋白表达缺失（32／40，80．0％），两种试验同时阴性的有31例（77．5％），另各有2例低表达。而在18例乳腺良性病变的患者，RT—PCR和western—blot均检测出14—3—3sigma基因表达。结论 14—3—3sigma基因表达缺失或低表达是乳腺癌的经常性事件，研究乳腺癌14—3—3sigma基因表达情况，可为乳腺癌的诊断提供实验室依据。

Expression of 14-3-3 sigma gene in patients with breast cancer and its clinical significance

Objective To investigate expression of 14-3-3 sigma gene in patients with breast cancer and its clinical significance.Methods Expression of 14-3-3 sigma gene was semi-quantitated by using RT-PCR and western-blot in 40 specimens of breast cancer and 18 specimens benign breast disease tissue.Results Of 40 cases of breast cancer 35(87.5%)were negative for 14-3-3 sigma gene in RT-PCR,32(80%)were negative in Western-blot,and 31(77.5%)were negative in both RT-PCR and western-blot.Besides,the expression in 2 cases was down-regulation in both the 2 method.In 18 specimens with benign breast disease tissue the expression of 14-3-3 sigma gene was detectable,which was demonstrated by RT-PCR or western-blot.Conclusion Inactivation and down-regulation of 14-3-3 sigma gene is a frequent event in breast cancer,and it may contribute to diagnosis of breast cancer.