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Application of Whole Mouse Embryo Culture System on Stem Cell Research |
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| Authors: | Kasem Kulkeaw Chiyo Mizuochi Yuka Horio Noriko Osumi Kohichiro Tsuji Daisuke Sugiyama | ||
| Institution: | (1) Faculty of Medical Sciences, Department of Hematopoietic Stem Cells, SSP Stem Cell Unit, Kyushu University, Fukuoka, Japan;(2) School of Medicine, Division of Developmental Neuroscience, Center for Translational and Advanced Animal Research, Tohoku University, Sendai, Japan;(3) Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, Sendai, Japan | ||
| Abstract: | The normal development of mouse embryo in vivo could be maintained in vitro up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O2 and CO2. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and
its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination
with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation
of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype
mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.
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| Keywords: | Mouse embryo Hematopoiesis Hematopoietic stem cell Whole embryo culture | ||
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