去甲万古霉素载药涤纶血管材料体外抗菌活性测定

目的：评价去甲万古霉素（NV）载药涤纶血管材料体外抗菌活性。方法：血管材料剪成直径6mm圆形。分别制成空白涤纶材料和载药（NV）涤纶材料，分别于0，7，14，28d取样观察体外缓释性能。选用耐甲氧西林表皮葡萄球菌（MRSE）和耐甲氧西林金黄色葡萄球菌（MRSA）作为试验菌，分别观察空白和载药涤纶血管材料0．7，14，28d和快慢相的抗菌效果。结果：两种细菌0，7，14，28d载药涤纶材料-细菌混合培养液的菌落数显著少于空白涤纶材料-细菌混合培养液（P均〈0．001）。结论：体外试验证实载NV涤纶材料MRSE和MRSA具有抗菌作用，且可持续维持28d。提示该载药材料具有持久抗菌活性，可用于制备抗感染血管移植物。

Measurement of in vitro antibacterial activity of norvancomycin-bonded Dacron prostheses

Objective:To evaluate in vitro antibacterial activity of norvancomycin-bonded Dacron prostheses.Methods:With the use of methicillin-resistant S.epidermidis(MRSE) and methicillin-resistant S.aureus (MRSA) as test organisms,culture of the mixture of a piece of 6-mm-diameter round prostheses (after soaking and shaking in PBS for 0,7,14 and 28 days),10ml broth containing 0.5ml bacterial suspension was performed to assess the quantity of bacteria grown,the colony-forming units (CFU) of staphylococcus were then plate-counted after culturing.Statistical analysis was completed using the paired t-test.Results:Norvancomycin-bonded Dacron prostheses produced significantly lower colony counts compared with unprotected Dacron prostheses for the two staphylococcus at 0,7,14 and 28 days.Conclusion:Norvancomycin-bonded Dacron prostheses exhibits persistent in vitro antibacterial activity against the two bacteria for up to 28 days.These results suggeste the norvancomycin-bonded Dacron prostheses as the promising infection-resistant vascular graft.