| ARG1基因的生物信息学和基因表达谱分析 |
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| 引用本文： | 顾永清,杨磊,潘泽民,袁红琳,谢毅. ARG1基因的生物信息学和基因表达谱分析[J]. 农垦医学, 2003, 25(2): 81-83 |
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| 作者姓名： | 顾永清 杨磊 潘泽民 袁红琳 谢毅 |
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| 作者单位： | 1. 石河子大学医学院,832002
2. 复旦大学生命科学院遗传学研究所基因分析组,200433 |
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| 基金项目： | 兵团医药专项基金资助项目 ,项目编号 :NKBO1SDXNK41YX |
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| 摘 要： | 目的 :对ARG1进行生物信息学和基因表达谱分析 ,并对其可能的抗砷机理进行探讨。方法 :对砷相关基因ARG1作进一步的生物信息学分析 ,用不同浓度砷染毒L - 0 2细胞两周以上 ,抽RNA转膜作Northern杂交 ,以及人类多组织膜的Northern杂交。结果 :Northern杂交结果显示该基因在未染毒L - 0 2细胞中几乎不表达 ,在砷染毒细胞中该基因的表达量明显增加 ,在 2 .3Kb处有一个单一的条带 ,且随剂量增加表达量亦增加。生物信息学分析显示ARG1具有一个液泡 -ATP酶 (Vacuolar -ATPase ,V -ATPase)样的功能域。结论 :ARG1是一条新的全长抗砷相关基因 ,其抗砷机理可能是通过液泡 -ATP酶介导的
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| 关 键 词： | 砷 基因 生物信息学分析 Northern杂交 |
Bioinformatic Analysis and Northern Blot of ARG1 Gene |
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| Gu Yongqing+ Yang Lei+ Pan Zemin+ Xie Yi+. Bioinformatic Analysis and Northern Blot of ARG1 Gene[J]. Agricultural Reclamation Medicine, 2003, 25(2): 81-83 |
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| Authors: | Gu Yongqing+ Yang Lei+ Pan Zemin+ Xie Yi+ |
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| Affiliation: | Gu Yongqing+1 Yang Lei+1 Pan Zemin+1 Xie Yi+2 |
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| Abstract: | Objective: Bioinformatic Analysis and Northern blot of arsenite relate gene1 (ARG1) and discuss on the possible mechanism of its arsenite resistant function. Method:Deeper bioinformatic analysis was performed on ARG1. Northern blot with the membrane derived from the RNA of different concentration arsenite induced L-02 cell was carried out to identify the previous result of cDNA microarray. Result:Northern blot shows ARG1 expressed higher in arsenite induced cell,furthermore its expression increased with the arsenite dosage.Bioinformatic analysis indicates ARG1 contains a domain of Vacuolar-ATPase. Conclusion:This gene might be a novel arsenite resistant gene and its arsenite resistant is through Vacuolar-ATPase. |
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| Keywords: | arsenite gene bioinformatic analysis Northern blot |
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