|
|||||
|
|
| 磁性纳米四氧化三铁对小鼠淋巴细胞及巨噬细胞功能影响的初步研究 | |
| 引用本文： | 金楠,王骏,陈宝安,丁家华,高冲,程坚,赵刚,鲍文,高峰,夏国华,周颖,陈月,周桂娜,李小毛,王雪梅.磁性纳米四氧化三铁对小鼠淋巴细胞及巨噬细胞功能影响的初步研究[J].中国实验血液学杂志,2010,18(1):136-139. |
| 作者姓名： | 金楠 王骏 陈宝安 丁家华 高冲 程坚 赵刚 鲍文 高峰 夏国华 周颖 陈月 周桂娜 李小毛 王雪梅 |
| 作者单位： | 1. 东南大学临床医学院附属中大医院血液科,江苏南京,210009 2. 萨尔大学物理系,德国萨尔布吕肯,D-66041 3. 东南大学生物电子学国家重点实验室,江苏南京,210096 |
| 基金项目： | 国家自然基金资助课题(编号30740062); 国家863课题资助项目(编号2007AA022007); 东南大学中大医院重大课题资助项目(编号2003YJ02) |
| 摘 要： | 为了探讨磁性纳米四氧化三铁颗粒(Fe3O4-MNP)对小鼠免疫系统的影响,将ICR小鼠随机分为4组,分别给予生理盐水、低剂量、中剂量和高剂量Fe3O4-MNP,在小鼠经胃灌药后2周处死,测量脾脏重量及小鼠体重并计算脾脏指数;用刀豆蛋白A(ConA)诱导的小鼠脾淋巴细胞转化试验测定脾淋巴细胞增殖活性,中性红比色法测定小鼠腹腔巨噬细胞吞噬功能。结果表明,与对照组相比,实验组的脾脏指数并没有明显差异(p0.05)。实验组脾淋巴细胞增殖率均高于对照组,尤其在中剂量组(p0.05),且中剂量组腹腔巨噬细胞吞噬率较对照组及其他2个实验组均有明显升高(p0.05)。结论:合适剂量的Fe3O4-MNPs可以增强小鼠淋巴细胞免疫和巨噬细胞吞噬功能。 |
| 关 键 词： | Fe3O4-MNPs 免疫功能 吞噬指数 脾细胞增殖 |
Influence of Magnetic Fe3O4 Nanoparticle on Functions of Lymphocytes and Macrophages in Mice |
|
| JIN Nan,WANG Jun,CHEN Bao-An,DING Jia-Hua,GAO Chong,CHENG Man,ZHAO Gang,BAO Wen,GAO Feng,XIA Guo-Hua,ZHOU Yin,CHEN Yue,ZHOU Gui-Na,LI Xiao-Mao,WANG Xue-Mei.Influence of Magnetic Fe3O4 Nanoparticle on Functions of Lymphocytes and Macrophages in Mice[J].Journal of Experimental Hematology,2010,18(1):136-139. | |
| Authors: | JIN Nan WANG Jun CHEN Bao-An DING Jia-Hua GAO Chong CHENG Man ZHAO Gang BAO Wen GAO Feng XIA Guo-Hua ZHOU Yin CHEN Yue ZHOU Gui-Na LI Xiao-Mao WANG Xue-Mei |
| Institution: | ( Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical College; Nanjing 210009, Jiangsu Province, China ; 1 Department of Physics, University of Saarland, D-266041 Saarbrueehen, Germany ;2 National Key Laboratory of Bioelectronies ( Chien-Shiung Wu Laboratory) , Southeast University, Nanjing 210096, Jiangsu Province, China) |
| Abstract: | This study was purposed to investigate the effects of magnetic nanoparticle of Fe3O4(Fe3O4-MNPs) on murine immune system.ICR mice were assigned randomly into four groups which were treated with normal saline,low,middle and high dose of MNP-Fe3O4 respectively.The mice were killed after being exposed by intragastric administration for 2 weeks.The ratios of spleen weight to body weight,lymphocyte transformation rate in spleen suspension and phagocytic index of macrophage in abdominal cavity were detected.The r... |
| Keywords: | magnetic nanoparticle of Fe3O4 immune function phagocytic index splenocyte proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |