1887份血培养病原菌分布及药物敏感性分析

目的 分析我院血培养标本各种病原菌分布情况及主要病原菌的耐药特点.方法 不同患者选择合适的标准需氧瓶、厌氧瓶,中和抗生索培养瓶及儿童专用培养瓶,采用BACT/ALERT3D全自动血培养仪对1887份血液标本进行血培养,分离所得菌株用MICROSCANPc12/NC21鉴定药敏系统进行鉴定和药敏试验.结果 培养时间缩短至5天,血培养的阳性率为8.2%(155/1887),155株阳性病原菌中革兰阳性球菌占43.2%(67/155),革兰阴性杆菌占48.4%(75/155),真菌占7.1%(11/155),革兰阳性杆菌占1.3%(2/155).最常见的病原菌是大肠埃希菌和金黄色葡萄球菌,未发现耐万古霉素的革兰阳性球菌,革兰阴性杆菌对碳青霉烯类抗菌素高度敏感.结论 全自动血培养仪提高了培养阳性率,缩短了培养时间,为血行性感染的早期诊断及合理用药提供实验依据.

Distribution and drug sensitivity test of pathogens in 1887 blood cultures

Objective To investigate the distribution and drug resistance of pathogens in blood cultures of clinical samples. Methods A total of 1887 samples of blood fluid were cultured by BACT/ALERT 3D automated blood culture system with standard aerobic vials, anaerobic vials, antibiotic-neutralized culture vials and peds plus vials performed for different patients, The isolated bacteria were identified by using the MICROSC AN Pc l2/NC21 system. Results Less than 5 days were expended for culture by the method of BACT/ALERT 3D.Of all the cultures, 155 strains of pathogens were isolated at a positive rate 8.2% (155/1887) ,of the 155 strains,43.2% were gram positive coccus (155/1887), 48.4% were gram negative bacilli (75/155), 1.3% were gram positive bacilli (2/155) and 7.1% were fungi (11/155). Data also showed that Escherichia coli and Staphylococcus aureus were dominant species whereas neither vancomycin-resistant gram positive coccus nor carbapenem-resistant gram negative bacilli were found. Conclusion The positive detection rate has been raised and the detection time reduced by BACT/ALERT 3D automated blood culture system, this result provided the evidence for early diagnosis and drug treatment against bloodstream infection.