三氧化二砷对肝癌HepG2细胞增殖的影响

目的　研究三氧化二砷 (As2 O3)对肝癌细胞的增殖抑制作用 .方法　应用台盼蓝拒染法、MTT比色法及细胞克隆形成试验 ,研究 As2 O3对肝癌 Hep G2细胞的增殖抑制和细胞毒作用 .结果　 As2 O3 能显著抑制肝癌细胞的生长 ,5μmol· L- 1 As2 O3 抑制程度明显强于 1μmol· L- 1 . 5和 1μmol· L- 1 As2 O3 作用后细胞克隆形成率分别为 (1.5±1.2 ) %和 (12 .3± 2 .2 ) % ,明显低于对照组的 (30 .0± 4.2 ) %(P<0 .0 1) .As2 O3对肝癌细胞有较强的细胞毒作用 ,且呈明显剂量和时间依赖性 ,其对肝癌细胞的杀伤率高于 5 -氟脲嘧啶 (5 - FU )和丝裂霉素 (MMC) ,但无显著性差异 ,As2 O3与 5 -FU及 MMC存在协同效应 ,联合应用杀伤率明显升高 .结论　 As2 O3具有明显的抑制肝癌细胞增殖作用 ,有必要进一步探讨其对肝癌的治疗价值

Arsenic trioxide curbs proliferation of liver cancer cell HepG2

AIM　To explore the inhibitive effect of arsenic trioxide (As2O3) on the proliferation of human liver cancer cell line-HepG2. METHODS　The morphologic changes, proliferation ability, colony-forming rate of HepG2 treated by As2O3 were studied with light microscopy, growth curve, trypan blue and colony-forming assay. The cytotoxicity of As2O3 on HepG2 was determined by MTT assay. RESULTS　Colony-forming rate of HepG2 with 5 μmol*L-1 As2O31.5±1.2)% was lower than that with 1 μmol*L-1 As2O3 (12.3±2.2)%, P<0.01 and much lower than that of control group (30.0±4.2)%, P<0.01. As2O3 had a remarkable cytotoxic effect on HepG2 and cytotoxic effect was dose-and-time dependent. The cytotoxic effect was enhanced through the combination of As2O3 with 5-Fu or MMC. CONCLUSION　As2O3 can significantly inhibit proliferation of HepG2 and so has a potential value of the clinical therapy on liver cancer.