p53、bcl-2及PCNA在新疆不同民族骨肉瘤中的异常表达及临床意义

目的：了解p53、bcl-2、PCNA基因在新疆不同民族骨肉瘤发生发展中的表达情况及临床意义。方法：应用免疫组化LSAB法检测52例骨肉瘤(哈族ll，维族17，汉族24例)和32例瘤样病损组织(骨纤维结构不良，纤维异常增殖症)的p53、bcl-2蛋白及PCNA的阳性检出情况。结果：p53、bcl-2蛋白、PCNA、在52例骨肉瘤中的检出率分别为42．31％(22／52)、59．62％(3l／52)、75％(39／52)，在骨的瘤样病变组织中(骨纤维结构不良，异常增殖症)的阳性检出率分别为3．13％(1/32)、0％(0/32)、31．25％(10/32)。它们的阳性率在骨肉瘤与骨的瘤样病变组织问均存在显著差异(P＜0．05)；哈、维、汉各民族之间p53、bcl-2、PCNA、阳性率的差别均无统计学意义(P＞0．05)；舌肉瘤中bcl-2与p53之间、bcl-2与PCNA之间有相关关系(P＜0．05)。结论：p53蛋白、癌基因bcl-2、PCNA相关基因的异常表达在骨肉瘤病变过程中具有重要作用；骨肉瘤组织中p53、bcl-2、PCNA表达在民族之间未见统计学差别。

BIOPATHOLOGICAL AND CLINICAL SIGNIFICANCE OF THE ABNORMAL EXPRESSION OF P53,BCL-2,PCNA IN OSTEOSARCOMAS TISSUES IN XINJIANG

Objective:To detect the abnormal expression and the relationships of p53 protein, bcl-2 oncogene , and proliferating cell nuclear antigene(PCNA)in osteosarcomas tissues in different ethnic patients, may involve the molecular biology mechanism of osteosarcomas carcinogenesis.Methods:By immunohistochemistry(LSAB methods ) we examined the over expression of p53, bcl-2 and PCNA on paraffin embedded tumor tissues in 52 different ethnic patients with osteosarcomas (Hazak11 ,Uygur 17, Han 24).Results:The positive frequency of p53, bcl-2 and PCNA in osteosarcomas was 42.31%(22/52), 59.62%(31/52),75.0%(39/52), respectively. And the expression of those in tumor-like lesions of bone was 3.13%(1/32),0%(0/32),31.25%(10/32). There were statistic significance between osteosarcomas and tumor-like lesions of bone ( P <0. 05).However, no difference of the expression of p53, bcl-2, PCNA among different ethnic was found ( P >0. 05).The significant correlationship was found between bcl-2 and PCNA, between bcl-2 and p53 ( P < 0.05).Conclusion: The abnormal expression of mutant p53, bcl-2 and PCNA may be an important etiopathogenesis factor for osteosarcomas carcinogenesis. No statistic difference in the expression of p53, bcl-2 and PCNA among different ethnic was found. The significant correlationship was found between bcl-2 and PCNA, between bcl-2 and p53. P53, bcl-2 and PCNA as oncogene and tumour suppress oncogene may play important roles in the development and prognosis of osteosarcomas.