| 甘蔗渣多糖脱蛋白方法研究 |
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| 引用本文： | 张斌,张璐,李沙沙,江晓,朱红霞,黄志刚.甘蔗渣多糖脱蛋白方法研究[J].辽宁中医药大学学报,2012(10):101-102. |
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| 作者姓名： | 张斌 张璐 李沙沙 江晓 朱红霞 黄志刚 |
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| 作者单位： | 南方医科大学中医药学院;九惠制药股份有限公司 |
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| 基金项目： | 广东省教育部产学研结合资助项目(2009B090300278);广州市白云区科技项目(2009-S2-41);广东省高校中药化妆品工程中心建设项目(GCZX-A1007) |
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| 摘 要： | 目的:比较Sevag法、酶法对甘蔗渣多糖脱蛋白的效果。方法:以蛋白脱除率、多糖含量为考察指标,选用Sevag法和酶法对甘蔗渣多糖进行脱蛋白处理。结果:进行8次Sevag法脱蛋白后蛋白的脱除率与多糖的含量分别达到77.47%、63.6%。酶法最佳条件下蛋白脱除率达到71.84%。结论:Sevag法进行8次脱蛋白对甘蔗渣多糖脱蛋白效果最佳;酶用量2%、温度50℃、时间3h对甘蔗渣多糖脱蛋白效果最佳。
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| 关 键 词： | 甘蔗渣 多糖 脱蛋白 正交实验 sevag法 酶法 |
Study on the Deproteinization Method of Polysaccharide in Sugarcane Residue |
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| ZHANG Bin,ZHANG Lu,LI Sha-sha,JIANG Xiao,ZHU Hong-xia,HUANG Zhi-gang.Study on the Deproteinization Method of Polysaccharide in Sugarcane Residue[J].Journal of Liaoning University of Traditional Chinese Medicine,2012(10):101-102. |
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| Authors: | ZHANG Bin ZHANG Lu LI Sha-sha JIANG Xiao ZHU Hong-xia HUANG Zhi-gang |
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| Institution: | 1.School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,Guangdong,China;2.Jiuhui Pharmaceutical Ltd.Huizhou 516007,Guangdong,China) |
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| Abstract: | Objective:To study the deproteinization by Sevag method and enzyme method of polysaccharide in Sugarcane Residue.Methods:Taking polysaccharide removal rate and polysaccharide content as criteria,deproteinization was treated by Sevag method and enzyme method.Results:After 8 times of deproteinization by Sevag method,polysaccharide removal rate was 77.47%,polysaccharide content was 63.6%.Polysaccharide removal rate was 71.84% by the optimum conditions of enzyme method.Conclusion:The deproteinization by Sevag method got to optimal effect after 8 times.The optimum conditions for enzyme method are as follows:enzyme concentration:2%;temperature:50℃;time:2h. |
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| Keywords: | sugarcane residue polysaccharide deproteinization orthogonal experiment Sevag method enzyme method |
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