序贯培养对小白鼠胚胎体外发育的影响

目的:探索序贯培养技术对小白鼠胚胎体外发育的影响。方法:将 2 -细胞期的小鼠胚胎随机分为序贯培养组与传统培养组进行培养 ,观察分析两组的 8-细胞期胚胎形成率、桑椹期胚胎形成率及囊胚形成率。结果:序贯培养组 8-细胞期胚胎形成率、桑椹期胚胎形成率及囊胚形成率分别为 70 .5 %、6 8.2 %、6 5 .9% ,传统培养组分别为 4 5 .7%、4 5 .7%、34.3%。两组比较 ,序贯培养组高于传统培养组 ,差异有统计学意义 (P <0 .0 5 )。结论 :采用适当的培养液进行序贯培养 ,更适合小白鼠胚胎的体外发育

Influence of sequential culture on development of mouse embryos in vitro

Objective: To investigate the effects of sequential culture technique on development of mouse embryos in vitro. Methods: 2 cell mouse embryos were allotted randomly to sequential culture media and conventional medium. To compare the 8 cell embryos' forming rate, morula forming rate and blastocyst forming rate between these 2 groups. Results: There were significantly higher 8 cell embryos, morula and blastocyst forming rate in sequential media group (70.5%, 68.2%, 65.9%) as compared with those in conventional medium group (45.7%, 45.7%, 34.3%). Conclusion: Sequential culture system is more suitable for the development of mouse embryos.