PTBP1通过EMT途径促进肝癌细胞的迁移与侵袭

目的:基于上皮-间充质转化(epithelial-mesenchymal transition,EMT)途径研究多聚嘧啶区结合蛋白1(polypyrimidine tract-binding protein 1,PTBP1)促进肝癌细胞迁移与侵袭的分子机制。方法:通过qPCR与Western blot实验筛选不同细胞中差异表达的剪接蛋白,生物信息学分析肝癌与正常肝组织中PTBP1的表达差异。划痕及侵袭实验研究过表达或敲减PTBP1对肝癌细胞迁移与侵袭能力的影响,Western blot检测过表达或敲减PTBP1对EMT通路蛋白的影响。结果:高转移肝癌细胞系HCCLM3中PTBP1的表达显著升高(P<0.05),且肝癌组织中PTBP1的表达水平明显高于正常组织(P<0.05)。过表达PTBP1可显著提高HCCLM3细胞的迁移与侵袭能力(P<0.05),并增加间充质标志物N-cadherin和vimentin等的表达(P<0.05),促进肝癌细胞的EMT进程。结论:PTBP1可通过促进肝癌细胞的EMT途径而促进肝癌细胞的迁移与侵袭。

PTBP1 promotes migration and invasion of liver cancer cells through EMT pathway

AIM:To investigate the molecular mechanism of polypyrimidine tract-binding protein 1(PTBP1)promoting the migration and invasion of hepatoma cells.METHODS:The differentially expressed splicing proteins in different cell lines were screened by qPCR and Western blot.The difference of the expression of PTBP1 between liver cancer and normal liver tissues was analyzed by bioinformatics.Wound-healing and Transwell assays were used to study the effect of PTBP1 on the migration and invasion of hepatoma cells,and Western blot was used to detect the effect of PTBP1 on epithelial-mesenchymal transition(EMT)signaling pathway.RESULTS:Compared with the HepG2 cells,the expression of splicing factor PTBP1 was significantly increased in the HCCLM3 cells with high metastatic ability(P<0.05),and the expression level of PTBP1 in hepatocellular carcinoma tissues was significantly higher than that in normal tissues(P<0.05).Over-expression of PTBP1 significantly increased the migration and invasion of HCCLM3 cells(P<0.05),increased the expression of mesenchymal marker proteins N-cadherin and vimentin(P<0.05),and promoted the EMT process of liver cancer cells.CONCLUSION:PTBP1 promotes the migration and invasion of liver cancer cells by promoting the EMT pathway of liver cancer cells.