NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum |
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| Authors: | L Xu B Xue L Zhou Z Qiu X Zhang N Xu Q Tang J Zhu X Guan Z Feng |
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| Institution: | 1. Department of Pathology, Nanjing Medical University, Nanjing, China;2. The Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, China;3. Department of Pathology, Northwestern University, Evanston, IL, USA;4. Huadong Medical Institute of Biotechniques, Nanjing, China |
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| Abstract: | The murine monoclonal anti‐idiotypic antibody, NP30, is a potential vaccine candidate against Schistosoma japonicum. Previous studies have revealed that NP30 has an immunoregulatory effect, but the underlying mechanism for this effect remains unknown. This study shows that NP30 induces dendritic cell (DC) maturation and increases the production of pro‐inflammatory cytokines. The expression of CD86 and MHC II was upregulated in DCs following stimulation with NP30 in vitro. Moreover, NP30 induced Th17 polarization by increasing the production of IL‐6 and TGF‐β. In vivo, Th17 differentiation was induced by the production of key pro‐inflammatory cytokines, including IL‐6and TGF‐β, from DCs of NP30‐immunized mice. These results indicate that NP30 promotes Th17 polarization through DC activation, preventing serious schistosomiasis. |
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| Keywords: | CD4 T lymphocytes dendritic cells schistosomiasis Th17 cells |
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