Similarity between Spatial-temporal Expression Patterning of HOXA11 and Progesterone Receptor in Human Endometrium

Objective To study HOXA 11 expression and its correlation with that of the progesterone receptor (PR) gene in human endometrium Methods In situ hybridization and semi-quantitative RT-PCR were used. Results HOXA 11 mRNA was detected in both stromal and glandular cells of normal endometrium by in situ hybridization. But the expression levels in the glandular cells had a dramatic decline or even disappearance at mid-secretory stage. Semi-quantitative RT-PCR analysis, however, demonstrated that the total expression levels of HOXA 11 mRNA were markedly increased in the mid-secretory endometrium, which suggested that there was an increased expression in stromal cells. Similar results were obtained for PR gene expression in human endometrium by in situ hybridization and semi-quantitative RT-PCR analysis, Conclusion HOXA 11 gene spatial and temporal e.vpression patterns were similar to that of PR gene in endometrium across menstrual cycle, and HOXA 11 was closely related to the endometrial proliferation and differentiation during menstrual cycle, especially the establishment of receptive status in implantation.

Similarity between Spatial-temporal Expression Patterning of HOXA11 and Progesterone Receptor in Human Endometrium

Objective To study HOXAll expression and its correlation with that of the progesterone receptor(PR) gene in human endometriumMethods In situ hybridization and semi-quantitative RT-PCR were used.Results HOXA l l mRNA was detected in both stromal and glandular cells of normal endometrium bv in situ hybridization. But the expression levels in the glandular cells had a dramatic decline or even disappearance at mid-secretory stage.Semi-quantitative RT-PCR analysis, however, demonstrated that the total expression levels of HOXAll mRNA were markedly increased in the mid-secretory endometrium, which suggested that there was an increased expression in stromal cells. Similar results were obtained for PR gene expression in human endometrium by in situ hybridization and semi-quantitative RT-PCR analysis.Conclusion HOXAll gene spatial and temporal expression patterns were similar to that of PR gene in endometrium across menstrual cycle, and HOXA l l was closely related to the endometrial proltferation and differentiation during menstrual cycle, especially the establishment of receptive status in implantation.