重组可溶性TRAIL联合顺铂诱导骨肉瘤MG-63细胞凋亡的研究

目的 研究重组可溶性TRAIL(rsTRAIL)与化疗药物顺铂 (CDDP)单独及联合使用诱导骨肉瘤细胞MG-63的凋亡作用.方法 常规培养骨肉瘤细胞系MG-63,用不同浓度rsTRAIL、CDDP和两药联用处理细胞,于处理后的不同时间观察细胞形态变化.应用四氮唑蓝(MTT)比色法和吖啶橙/溴化乙锭(AO/EB)双重荧光染色法检测两药单独使用和联合使用诱导骨肉瘤MG-63细胞凋亡的作用.结果 rsTRAIL对MG-63细胞的生长有明显的抑制作用,可见典型的细胞凋亡特点,细胞抑制率从6 μg/L的10.1%上升到800 μg/L的84.6%,细胞凋亡率从3 μg/L的19%提高到25 μg/L的69.7%,均具显著的剂量效应;亚毒性剂量的CDDP与低浓度的rsTRAIL合用,明显提高了MG-63细胞的生长抑制率和凋亡率(P＜0.05).结论 rsTRAIL具有诱导骨肉瘤MG-63细胞凋亡的作用,并通过诱导凋亡的方式有效地杀伤肿瘤细胞;化疗药物CDDP能加强rsTRAIL的抗肿瘤活性.

Apoptosis-inducing Effect of rsTRAIL and Cisplatin on Cancer Cell Lines MG-63

Objective To study the apoptosis-induced effect of the recombinant solubility TRAIL(rsTRAIL) on cell lines MG-63,and investigate the combination anti-tumor effect of rsTRAIL and cisplatin(CDDP). Methods The morphology of cultured cell treated with or without rsTRAIL was observed and compared microscopically.To explore the methods to increase the sensitivity to TRAIL,cancer cell lines MG-63 were treated with lower dosage of rsTRAIL and sub-toxicity level CDDP.The combination anti-tumor effect determined by MTT assay and AO/EB double fluorescence staining were compared with that in control groups treated with single TRAIL or CDDP. Results Cells killed by rsTRAIL show typical apoptosis morphology changes.The recombinant TRAIL had significant anti-proliferative effects on MG-63 cells.The anti-proliferative rate was 10.1% to 84.6%,and the apoptosis rate was 19% to 69.7%.The classic dose-effect relationship was founded.The sub-toxicity level CDDP can significantly increase the sensitivity of MG-63 to the recombinant TRAIL.Treated with CDDP and TRAIL,the apoptosis rate of MG-63 reached 81%. Conclusion The recombinant TRAIL can kill MG-63 cells by inducing cells apoptosis.CDDP can synergitically enhance the effect of the recombinant TRAIL on inducing apoptosis in A549 and MG-63 cells.