| HIV-1 p24抗原检测的应用研究 |
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| 引用本文： | 尹红章,李秀华,孟淑芳,宋爱京,李德富. HIV-1 p24抗原检测的应用研究[J]. 中国免疫学杂志, 2000, 16(3): 151-152,154 |
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| 作者姓名： | 尹红章 李秀华 孟淑芳 宋爱京 李德富 |
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| 作者单位： | 中国药品生物制品检定所卫生部生物技术产品检定及标准化研究重点实验室,北京,100050 |
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| 基金项目： | 国家“九五”科技攻关资助!( 96 C0 2 0 1 16) |
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| 摘 要： | 目的：用抗ＨＩＶ－１ｐ２４单克隆抗体和多克隆抗体和多克隆抗体构建的间接ＥＬＩＳＡ试剂，检测ＨＩＶ－１抗体阳性及其它样品，探讨应用的可行性。方法：采用单克隆抗体固相、兔抗ＨＩＶ－１ｐ２４抗体夹心，羊抗兔ＨＲＰ结合物的间接ＥＬＩＳＡ法。结果：显示试剂盒ＨＩＶ－１ｐ２４抗原检出灵敏度为５０ｐｇ／ｍｌ（基因工程抗原）；特异性９９．４６％；ＨＩＶ抗体阳性血样性率３．２％；抗体阴性的特殊人群血样阳性率３．９＆
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| 关 键 词： | HIV－1p24 ELISA 单克隆抗体 艾滋病 |
Prevalence study of HIV-1 p24 antigen detection kit in different samples |
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| YIN Hong Zhang,LI Xiu Hua,MENG Shu Fang et al. Prevalence study of HIV-1 p24 antigen detection kit in different samples[J]. Chinese Journal of Immunology, 2000, 16(3): 151-152,154 |
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| Authors: | YIN Hong Zhang LI Xiu Hua MENG Shu Fang et al |
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| Affiliation: | YIN Hong Zhang,LI Xiu Hua,MENG Shu Fang et al. The Key Lab.of Ministry of Public Health for the Control and Standard Research of Biotechnic Products,National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050 |
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| Abstract: | Objective: The indirect Sandwich ELISA kit for detection of HIV 1 p24 antigen were constructed to test different kind of samples.Methods: Sensitivity and specificity of the kit were 50 pg/ml and 99.46% respectively. Among the different samples 3.2% of anti HIV positive,3.9%(6/152) of high risk group populations and 15.4%(4/26) of anti HIV indeterminant samples which were shown HIV 1 p24 positive. Results:The detection of virus culture supernatant also showed the high sensitivity of the test kit.Conclusion: The HIV 1 p24 test kit will be useful for detection of HIV infection followed anti HIV screening and for virological study. |
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| Keywords: | HIV 1 p24 ELISA McAb |
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