芩连合剂质量标准研究

目的建立芩连合剂的质量标准：方法采用薄层色谱法对处方中金银花、连翘、黄芩进行定性鉴别，用反相高效液相色谱（RP—HPLC）法对处方中的黄芩进行含量测定。色谱柱为Dikma C18柱（250mm×4．6mm，5um），流动相为甲醇-水-冰醋酸（50：50：1），检测波长为274nm，柱温25℃，流速1．0mL／min。结果金银花、连翘的薄层色谱分离良好，斑点明显，专属性好，无干扰；黄苓苷进样量在0．1186～0．9488ug范围内与峰面积线性关系良好（r=0．9999），平均回收率为98．8％，RSD=0．47％（n=6）。结论所用方法简便、准确、重现性好，可作为芩连合别的质量控制方法。

Study on Quality Standard of Qinglian Mixture

Objective To establish the quality standard of Qinglian Mixture. Methods TLC was used to identify qualitatively Flos Lonicerae, Forsythia Suspense, Radix Scutellariae. Baicalin in the formula was detected by RP-HPLC. The chromatographic column was Dikma CI8 （250 mm × 4.6 mm,5um）. The mobile phase was methanol -water- acetic acid （50 ： 50 ： 1）. The detection wavelength at 274 nm. The temperature was 25℃ with the flow rate of 1.0 mL/min. Results The thin layer chromatographic separation of Flos Lnoicerae, Forsythia suspense ,was better with obvious spots, good specificity and without interference. The linear range of baiealin was 0. 118 6-0. 948 8 ug（ r= 0. 999 9）. The average recovery was 98.80%, and RSD was 0. 47%（n = 6）. Conclusion The method is simple and aceurate with good reproducibility. It can be used for the quality control of Qinglian Mixture.