EVn-50对人宫颈癌Hela细胞增殖和凋亡的影响

目的:研究EVn-50体外对人宫颈癌Hela细胞增殖及凋亡的影响。方法:体外培养Hela细胞,台盼蓝拒染法检测细胞活力;AO/EB染色荧光显微镜观察EVn-50诱导Hela细胞凋亡形态学改变;DNA凝胶电泳确证EVn-50诱导Hela细胞凋亡作用;PI染色流式细胞仪检测EVn-50诱导Hela细胞凋亡率。结果:EVn-50体外对人宫颈癌Hela细胞的增殖具有显著抑制作用,呈浓度依赖性。EVn-50可诱导Hela细胞凋亡,AO/EB染色可见典型凋亡小体;DNA凝胶电泳在EVn-50浓度100μg/mL作用48h出现典型"梯形"DNA条带;PI染色流式法显示EVn-50在10、100μg/mL作用Hela细胞48h后,凋亡率分别为(8.80±0.16)%及(20.93±0.62)%,呈浓度依赖性。结论:EVn-50具有抑制人宫颈癌Hela细胞增殖并诱导细胞凋亡作用。

Effect of EVn-50 on proliferation and apoptosis of the human cervical carcinoma cell line Hela

Objective To investigate the effect of EVn-50 on the proliferation and apoptosis of human cervical carcinoma (Hela) cell line in vitro. Methods Hela cells were cultured in vitro. The trypan blue exclusion method was used to determine the effect of EVn-50 on the proliferation of Hela cells. AO/EB fluorescence staining was used to observed the morphologic changes of apoptosis induced by EVn-50 in Hela cell line. DNA agarose gel electrophoresis was used to test apoptosis induced by EVn-50 in Hela cell line . Flow cytometry using PI staining was used to examine apoptosis on cervical carcinoma Hela cell lines. Results EVn-50 significantly inhibited human cervical carcinoma Hela cell lines proliferation in vitro in a dose-dependent manner. Typical morphologic changes of apoptosis could be observed after treatment with EVn-50 by fluorescence microscope using AO/EB fluorescence staining. DNA agarose gel electrophoresis shown that DNA ladder bands could appear after treatment with EVn-50 at 100μg/mL for 48h.PI stainning flow cytometry(FCM) analysis indicated that the apoptosis rate of Hela cells was (8.80±0.16)% and (20.93±0.62)% respectively after treatment with EVn-50 using the concentrations of 10、100μg/mL for 48h (P＜0.05). EVn-50 induced Hela cells apoptosis in a dose-dependent manner. Conclusions EVn-50 can significantly inhibit Hela cells proliferation and induce apoptosis.