胃癌线粒体基因组控制区变异和不稳定性的研究

目的 :检测胃癌线粒体基因组的控制区 (controlregion)的不稳定性、多态性及其他变异 ,探讨其与胃癌的关系。方法 :对2 2例胃癌患者的癌组织及其癌旁正常组织共 44个样本的线粒体DNA (mitochondrialDNA ,mtDNA)的控制区通过PCR扩增 ;然后通过直接测序检测控制区的不稳定性、多态性以及其他变异。结果 :mtDNA控制区不稳定性在胃癌组织和癌旁正常黏膜组织间无明显差异 ,分别为 5 9 1% ( 13 /2 2 )和40 9% ( 9/2 2 ) ,P =0 3 7;并且与胃癌分化程度也不相关 ,P =0 42。 7例样本显示np16182PolyC不稳定性 ,其中 5例伴有np16189T C的转换。另外研究还发现了一些新的控制区变异。结论 :线粒体DNA控制区变异和不稳定性对胃癌发生的作用有限。np165 69T C的转换很可能是np 16182PolyC不稳定性的重要原因之一

Study on variations and instabilities of mitochondrial control region in gastric cancer

OBJECTIVE: To detect the instabilities, polymorphisms and other variations of mitochondrial DNA (mtDNA) control region in gastric cancers, and study the relationships between them and gastric cancer.METHODS:mtDNA control region was detected for instabilities, polymorphisms and other variations via PCR amplification followed by direct DNA sequencing in 22 matched gastric cancerous and paracancerous tissues. RESULTS:There was no statistic significance in variations of mtDNA control region between gastric cancerous tissues and matched paracancerous tissues, 40.9% (9/22) and 59.1% (13/22) respectively, P=0.37, nor was there between highly differentiated gastric cancers and the lowly differentiated gastric cancers, P=0.42. np16182 polyC instabilities were found in seven samples, five of which simultaneously had a np 16 189 T-C transition. Some new variations were found. CONSLUSIONS:There is no relationship between gastric cancerigenesis and variations or instabilities of mtDNA control region. np 16 189 T-C transition may be an important factor of polyC instability in gastric cancer.