端粒保护蛋白过表达对HeLa细胞染色体端-端融合和端粒酶活性的影响

目的 观察人端粒保护蛋白1（human protection of telomeres1，hPOT1）基因过表达对HeLa细胞染色体端-端融合和端粒酶活性的影响。方法 利用先前构建的hPOT1基因真核表达重组质粒pcDNA3-hPOT1，经脂质体介导瞬时转染HeLa细胞介导hPOT1基因过表达；秋水仙碱阻滞细胞周期于分裂中期，DAPI荧光染色分析染色体端-端融合；Telomerase PCR-ELISA试剂盒检测端粒酶活性。结果 pcDNA3-hPOT1重组质粒转染HeLa细胞5d后，染色体端-端融合几率增加，对端粒酶活性无明显影响。结论 提示hPOT1蛋白可导致染色体端-端融合。

Effects of hPOT1 overexpression on chromosome end-to-end fusions and telomerase activation of HeLa cells

Objective To observe the effects of hPOT1 overexpression on chromosome end-to-end fusions and telomerase activation in HeLa cells. Methods By an eukaryotic expression plasmid of pcDNA3-hPOT1 constructed preliminarily, liposome mediated transient transfection of HeLa cells and hPOT1 overexpression. Cell cycles were blocked in metaphase by colchicina. Chromosome end-to-end fusions were analyzed by DAPI fluorescein stain. Telomerase PCR-ELISA kit was used to determine telomerase activations. Results After 5 days of the pcDNA3-hPOT1 recombinant plasmids transfected into HeLa cells, the probabilities of chromosome end-to-end fusions inereased. And there was no obvious effect on telomerase activation. Conclusions hPOT1 may induce chromosome end-to-end fusions.