激光诱导药物诱发肝癌小白鼠血清荧光光谱分析

目的 研究药物诱发肝癌小白鼠的血清荧光光谱。方法 用532nm激光诱导荧光，多功能光栅光谱仪获得药物诱发肝癌小白鼠的血清荧光光谱。结果 肝癌小白鼠与健康小白鼠血清荧光光谱在586nm和708nm附近都有2个锐峰，但肝癌小白鼠血清荧光光谱的这2个锐峰蓝移了约2nm；健康小白鼠血清荧光光谱在这2个锐峰之间没有谱峰，而肝癌小白鼠的血清荧光光谱在这2个锐峰之间有2个明显的谱峰，分别在615nm和643nm处，且强度较大。结论 肝癌小白鼠的血清荧光光谱有很强的特异性。

The laser-induced fluorescence spectrum of blood serum from the mouse bearing drug-induced liver cancer

Objective To study the fluorescence spectrum of the serum from the mice bearing drug-induced liver cancers (H22).Methods The fluorescence was induced with 532 nm laser. The fluorescence spectrum was acquired and displayed on the multifunction grating spectrometer.Results There were two razor-edges by 586 nm and 708 nm in the spectra both of the liver- cancer mice and of the healthy mice, but the two razor-edges of the former ahowed a slight blue shift for about 2 nm. As a rule, there were no spectral summits between these two razor-edges in the case of healthy mouse; however, there were two definite spectral summits located at 615 nm and 643 nm in the fluorescence spectrum of liver- cancer mouse serum, characterizing the presence of cancer.Conclusion The fluorescence spectrum of liver-cancer mouse serum has obvious characteristics.