CIN和子宫颈鳞癌的HPV16/18感染与p53、MDM2蛋白表达的关系

目的研究子宫颈鳞癌及C IN的HPV感染、p53蛋白和M DM 2蛋白的表达及三者之间的相互关系。方法选取子宫颈鳞癌61例、C IN 47例、正常子宫颈组织54例,分别采用原位杂交技术检测HPV 16/18感染,用免疫组化技术S-P法检测p53蛋白和M DM 2蛋白。结果HPV 16/18感染率鳞癌组为62.3%(38/61),C IN组为70.2%(33/47),对照组为16.7%(9/54)。C IN组和鳞癌组的HPV 16/18的感染率均显著高于对照组(P值均<0.05)。而C IN组与鳞癌组的HPV 16/18感染率差异无显著性(P>0.05)。C IN组、鳞癌组、对照组的p53阳性率分别为19.1%(9/47)、26.2%(16/61)、3.7%(2/54)。C IN组、鳞癌组、对照组的M DM 2阳性率分别为14.9%(7/47)、26.2%(16/61)、7.4%(4/54)。C IN组和鳞癌组的p53阳性率均显著高于对照组(P值均<0.05),C IN组与鳞癌组比较差异无显著性(P>0.05)。M DM 2阳性率鳞癌组显著高于对照组(P<0.05),而在C IN组和鳞癌组之间、C IN组和对照组之间差异均无显著性(P值均>0.05)。在本实验各组中,p53与M DM 2之间的阳性率均无统计学相关(P值均>0.05)。p53阳性率和HPV 16/18感染之间差异无显著性(P值均>0.05),而M DM 2的阳性率与HPV 16/18感染仅在子宫颈鳞癌组呈正相关(P<0.05)。结论C IN及子宫颈鳞癌与HPV 16/18感染关系密切;p53和M DM 2均参与子宫颈癌及癌前病变的发生,但p53的突变、M DM 2的扩增和过表达均不占主导地位。

p53 and MDM2 expression with HPV16/18 infection in human uterine cervical carcinoma and cervical intra-epithelial neoplasia

Objective To investigate the expression of p53 and MDM2 protein,and HPV16/18 infection in squamous cervical carcinoma(SCC) and cervical intraepithelial neoplasia(CIN).Methods HPV16/18 were detected by in situ hybridization(ISH),the expression of p53 and MDM2 protein was examined by immunohistochemistry in 61 of SCC,47 of CIN and 54 of normal cervix(NC).Results HPV16/18 positive rates in SCC,CIN and NC groups were 62.3%(38/61),70.2%(33/47) and 16.7%(9/54),respectively(SCC vs NC and CIN vs NC,P<0.05).The p53 positive rates in SCC,CIN and NC groups were 26.2%(16/61),19.1%(9/47) and 3.7%(2/54),respectively(SCC vs NC and CIN vs NC,P<0.05;SCC vs CIN,P>0.05).The MDM2 positive rates in SCC,CIN and NC groups were 26.2%(16/61),14.9%(7/47) and 7.4%(4/54),respectively(CIN vs NC,P>0.05;SCC vs NC and SCC vs CIN,P<0.05).There was no correlation among the positive rates of p53 protein or MDM2 protein with HPV16/18 infection rates(P>0.05),while the MDM2 positive rates was related to HPV16/18 infection rates in SCC group(P<0.05).Conclusions HPV16/18 infection has a strong association with SCC and CIN.p53 and MDM2 proteins may be involved in the development of CIN and SCC.