输血后感染的HIV-1流行株分子流行病学调查

目的了解河北省沙河市受血感染者及其夫妻传播者中HIV-1亚型特点和来源。方法采集HIV-1感染者的全血样品,分离外周血单核细胞(PBMC),提取前病毒DNA,使用套式聚合酶链反应(nested-PCR),扩增HIV-1的env基因的C2～V3区,并进行序列测定和亚型分析。结果对7例HIV-1感染者的血标本进行nested-PCR扩增,得到6份HIV-1 env C2～V3基因片段,经序列测定分析,6份样品均为B′亚型。组内基因离散率6.22%±1.38%,基因序列与云南瑞丽株RL42(泰国B亚型)相近。受血者Heb0224及其配偶Heb0223与云南瑞丽株RL42 env的基因离散率分别为3.93%和7.71%。结论在河北省沙河市受血感染者及其夫妻传播者中流行的为HIV-1 B′亚型。

Molecular epidemiological investigation of HIV-1 circulating strain infected after blood receiving

Objective To understand the characteristics and origin of subtypes of HIV-1 strains infected blood receivers and their couples in Shahe,Hebei Province.Methods The whole blood from HIV-1 infected individuals were collected and the peripheral blood mononuclear cell were separated.The provirus DNA was extracted and amplified by nested-PCR,after which the HIV-1 env C2～V3 gene fragments was sequenced and its subtypes were analyzed.Results Among 7 HIV-1 antibody positive cases,6 HIV1 env C2～V3 gene fragments were amplified and analyzed.6 cases were confirmed to be infected with HIV-1subtype B′ strain.The genetic distance of HIV-1 B′ intersubtype was 6.22%±1.38%（n=6）,their gene sequence were closely related to RL42 in Yunnan（Thailand B）.To compare with RL42, the genetic distance of blood receiver（Heb0224） was 3.39% and that of their spouses（Heb0223） was 7.71%.Conclusions HIV-1 subtype B′ was the predominant subtype circulating among blood receivers and their spouses within families in Shahe,Hebei Province.