caspase 3在细胞因子调节急性白血病细胞凋亡中的变化

目的 研究ｃａｓｐａｓｅ３在粒细胞集落刺激因子（Ｃ－ＣＳＦ）和粒巨洚细胞集落因子（ＧＭ－ＣＳＦ）调节白血病细胞系ＮＢ４细胞凋亡过程中的变化及其意义。方法 利用细胞这、流式细胞仪分析及ＤＮＡ片段化比率测定，检测Ｇ－ＣＳＦ及ＤＭ－ＣＳＦ对ＮＢ４细胞凋亡的影响，应用荧光分光光度法检测ｃａｓｐａｓｅ３活性变化，并利用ＡＥ－ＤＥＶＤ－ＣＲＯ进行ｃａｓｐａｓｅ３抑制试验。结果 Ｇ－ＣＳＦ能诱导ＮＢ４细胞凋亡，

Caspase 3 in the cytokines induced acute leukemia cell apoptosis

Objective To explore the variation and significance of caspase 3 activity in the process of G CSF and GM CSF regulated NB4 cell apoptosis. Methods Cell morphology, flow cytometry and DNA fragmentation assay were used to demonstrate the apoptosis induced by G CSF and GM CSF. Caspase 3 activity was detected by spectrofluorometry,and its inhibition test by AC DEVD CHO. Results G CSF could induce NB4 cell apoptosis,while GM CSF could not. Caspase 3 activity was significantly higher in the apoptotic cells than in control cells. The G CSF induced NB4 cell apoptosis was efficiently inhibited by AC DEVD CHO. Conclusions G CSF and GM CSF exert different effects on the apoptosis of leukemic cells. G CSF induced apoptosis of leukemic cells through activation of caspase 3.