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| 早期糖尿病NOD小鼠视网膜HIF-1的表达 | |
| 引用本文： | 李才锐,胥延,姜德咏,洪卫,郭小健,王平,李雯霖. 早期糖尿病NOD小鼠视网膜HIF-1的表达[J]. 眼科学报, 2006, 22(2): 107-111 |
| 作者姓名： | 李才锐 胥延 姜德咏 洪卫 郭小健 王平 李雯霖 |
| 作者单位： | 1. 中南大学湘雅二医院眼科,长沙,410007;大理医学院附属医院眼科,大理,671000 2. 昆明医学院附属第二医院眼科,昆明,650101 3. 中南大学湘雅二医院眼科,长沙,410007;湖南博雅眼科医院,长沙,410007 4. 大理医学院附属医院眼科,大理,671000 5. 中南大学湘雅二医院眼科,长沙,410007 |
| 基金项目： | 湖南省科技厅基金资助项目(04SK3034/3) |
| 摘 要： | 目的:探讨糖尿病NOD小鼠视网膜HIF-1α的表达。方法:NOD小鼠随机分为正常对照组和糖尿病组,分别于糖尿病2、4、6、8和12周处死正常对照组和糖尿病组小鼠,摘除眼球后分离视网膜。Westernblot方法检测视网膜HIF-1α的表达,免疫组化方法检测视网膜上HIF-1α的表达。结果:与正常对照组相比,糖尿病组小鼠血糖明显增高,体重明显下降(P<0.01);Westernblot显示糖尿病6周时视网膜HIF-1α低水平表达,12周时HIF-1α表达增高;免疫组化显示糖尿病组12周时视网膜内层HIF-1α表达增加,而未见视网膜外层HIF-1α表达。结论:糖尿病早期视网膜HIF-1α表达增高,说明在糖尿病早期视网膜发生缺血缺氧;HIF-1α可能参与了糖尿病早期视网膜缺血缺氧的继发损害过程。 |
| 关 键 词： | 视网膜 低氧诱导因子-1α |
The Expression of HIF-1 in the Early Diabetic NOD Mice |
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| Cairui Li,Yan Xu,Deyong Jiang,Wei Hong,Xiaojian Guo,Ping Wang,Wengling Li. The Expression of HIF-1 in the Early Diabetic NOD Mice[J]. Eye science, 2006, 22(2): 107-111 | |
| Authors: | Cairui Li Yan Xu Deyong Jiang Wei Hong Xiaojian Guo Ping Wang Wengling Li |
| Affiliation: | 1. Department of Ophthalmology, Xiangya Second Hospital of Central South University, Changsha 410007 China;2. Department of Ophthalmology, the Affiliated Hospital of Dali Medical College, Dali 671000, China;3. Department of Ophthalmology, Second Hospital of Kuming Medical College, Kunming 650101 China;4. Hunan Boya Ophthalmology Hospital, Changsha 410007, China |
| Abstract: | PURPOSE: To study the retinal expression of HIF-1 alpha in the diabetic NOD mice. METHODS: NOD mice were divided into normal control group and diabetes group randomly, sacrificed at 2, 4, 6, 8 or 12 weeks. Eyes were enucleated and retinas were isolated and used for western blot, or eyes were sectioned for immunohistochemical staining for HIF-1 alphal. RESULTS: Compared to the normal control group, blood sugar of diabetes mice increased significantly but the weights decreased significantly (P < 0.01). Western blot of retinal lysates showed low levels of HIF-1 alpha at the 6th weeks that were markedly increased at the 12th weeks in the diabetes group. Immunohistochemistry showed increased staining for HIF-1 in the inner retina, but not in the outer retina. CONCLUSION: The retinal HIF-1 alpha levels increasing showed that retinal ischemia happening in the early diabetes. HIF-1 alpha may participate in the secondary damage of retinal ischemia induced by early diabetes. |
| Keywords: | Retina HIF-1 |
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