The use of the mechanical fragility test in evaluating sublethal RBC injury during storage |
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| Authors: | J S Raval J H Waters A Seltsam E A Scharberg E Richter A R Daly M V Kameneva M H Yazer |
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| Institution: | 1. Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA;2. Department of Anesthesiology, The Magee Womens Hospital of the University of Pittsburgh Medical Center, Pittsburgh, PA, USA;3. Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA;4. German Red Cross Blood Service NSTOB, Institute Springe, Springe, Germany;5. Institute for Transfusion Medicine and Immunohematology, German Red Cross Blood Service Baden‐Württemberg – Hessen, Baden‐Baden, Germany;6. McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA;7. Department of Surgery, Pittsburgh, PA, USA;8. The Institute for Transfusion Medicine, Pittsburgh, PA, USA |
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| Abstract: | Background The mechanical fragility index (MFI) is an in vitro measurement of the extent of RBC sublethal injury. Sublethal injury might constitute a component of the RBC storage lesion, thus the MFI was determined serially during routine RBC storage. Methods Leucoreduced AS‐5‐ and SAGM‐preserved RBCs were stored under routine blood bank conditions. The mechanical fragility (MF) of each unit was serially measured during storage. Results For both AS‐5 and SAGM units, male and female RBCs demonstrated statistically significant increases in the MFI during storage. The MFI was significantly lower in AS‐5 units compared to SAGM units throughout storage. Female RBCs had significantly lower MFI vs. male RBCs in both AS‐5 and SAGM units at all times. No significant differences in MFI were observed between ABO groups for both genders for AS‐5 RBCs. Conclusions The MF of RBCs increases during storage. Both gender and preservation solution influenced the MFI; however, the male:female MFI ratios were similar at all time‐points and remained stable, suggesting that gender‐based biological differences exist independent of storage solution. The MF could be a useful test for evaluating the effect of novel interventions intended to mitigate the susceptibility of RBCs to sublethal injury during storage. |
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| Keywords: | AS‐5 haemolysis lesion mechanical fragility RBC SAGM storage |
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