The invasive behavior of two human nasopharyngeal carcinoma (NPC) cell lines CNE-1, CNE-2Z in vitro]

Method of co-culture of tumor cells with precultured heart fragments (PHF) in vitro was used in studying the invasive behavior of human nasopharyngeal carcinoma (NPC) cell lines CNE-land CNE-2Z respectively. This improved method was based on the gyrotory shaker organ culture technique originally established by Mareel in order to show NPC cell invasiveness in a rather identical image. Histopathological sections revealed that the tumor cells might invade into the cell layer of fibroblasts surrounding the heart tissue. This would make the heart muscle cells degenerated, atrophied and necrotic and finally displaced by the fibrotic tissue. Data also pointed out that CNE-2Z cells showed more active invasive ability than that of the CNE-1 cells in the organ culture.