慢性阻塞性肺疾病患者支气管肺泡灌洗液中HMGB1的表达及意义

目的：通过测定慢性阻塞性肺疾病（COPD）患者的支气管肺泡灌洗液（BALF）中高迁移率族蛋白B1（HMGB1）的含量并分析 HMGB1和气流受限的关系，初步探讨 HMGB1在COPD发病机制中的作用。方法将研究对象分为COPD组（COPD稳定期20例）和对照组（上气道咳嗽综合征20例），均行支气管镜检查并行支气管肺泡灌洗术，检测并比较两组BALF中的细胞总数、中性粒细胞百分比；用ELISA法测定并比较两组 HMGB1、白细胞介素‐1β（IL‐1β）及肿瘤坏死因子‐α（TNF‐α）的含量，分析COPD组 HMGB1和IL‐1β、TNF‐α间的关系及 HMGB1和肺功能（FEV1％预计值）的关系。结果在BALF中，COPD组细胞总数、中性粒细胞百分比高于对照组（P＜0．01）；COPD组HMGB1、IL‐1β、TNF‐α的含量高于对照组（P＜0．01、P＜0．05、P＜0．01），COPD组 HMGB1的含量和IL‐1β、TNF‐α呈正相关（r＝0．79，P＜0．01及 r＝0．48，P＜0．05）；COPD组 HMGB1和肺功能值（吸入支气管舒张剂后FEV1％预计值）呈负相关（r＝－0．70，P＜0．01）。结论 HMGB1参与和促进COPD气道炎症的发生、发展，BALF中HMGB1水平和COPD严重程度相关。

Expression and significance of high mobility group box 1 protein in BALF of patient with COPD

Objective To preliminarily investigate the effect of high mobility group box 1 (HMGB1) in the pathogenesis of chronic obstructive pulmonary disease (COPD) by detecting the HMGB1 level in bronchoalveolar lavage fluid(BALF) of the COPD patients and analyzing the relation between HMGB1 and airway limitation .Methods The research subjects were divided into the COPD group (stable COPD ,20 cases) and control group20 cases of upper airway cough syndrome(UACS)] .All subjects were performed the bronchoscopic examination as well as bronchoalveolar lavage .The total cell counting and percentage of neutrophilic granulocytes in BLAF were detected and compared between the two groups .The HMGB1 ,IL‐1βand TNF‐αlevels in BLAF were detected by using ELISA and compared between the two groups .The relation between HMGB1 with IL‐1βand TNF‐αand the rela‐tion between HMGB1 with pulmonary function(FEV1% predicted value) in the COPD group was analyzed .Results The total cell counting and percentage of neutrophilic granulocytes of BLAF in the COPD group were higher than those in the control group (P<0 .01) .The levels of HMGB1 ,IL‐1β,TNF‐αof BALF in the COPD group were higher than those in the control group(P<0 .01 , P<0 .05 ,P<0 .01) .In the COPD group ,the HMGB1 level in BALF was positively correlated with the IL‐1βand TNF‐αlevels(r=0 .79 ,P< 0 .01 ;r= 0 .48 ,P< 0 .05) ,while negatively related with the pulmonary function value(FEV1% predicted value ,r=-0 .70 ,P<0 .01) .Conclusion HMGB1 participates in and promotes the occurrence and development of COPD airway inflamma‐tion and the HMGB1 level in BALF is correlated with the severity of COPD .