迈瑞BC-6800测定MCV、MCH及MCHC参考区间的调查

目的：调查迈瑞BC‐6800测定健康成年人静脉血红细胞平均体积（MCV）、平均红细胞血红蛋白含量（MCH）及平均红细胞血红蛋白浓度（MCHC）的参考区间，以建立适合广东省中医院检验科的参考区间。方法参照美国临床实验室标准化委员会（NCCLS）C28‐A3文件推荐方法，选取600例健康成年人作为参考个体，采用迈瑞BC6800进行MCV、MCH及MCHC测定，构建参考区间；另选取150例健康体检者，收集MCV、MCH、MCHC检测值，对所建立的参考区间进行验证。结果健康成人MCV、MCH及MCHC检测结果均呈正态分布。各年龄段MCV比较，差异有统计学意义（P＜0．05）；参考区间：青年82．278～94．242fL、中年83．032～94．608fL、老年83．137～96．343fL。不同性别及各年龄段MCH比较，差异均有统计学意义（P＜0．05）；参考区间：男性青年27．785～32．415pg、男性中年28．324～32．456pg、男性老年28．274～32．966pg，女性青年27．367～31．973pg、女性中年27．445～32．215pg、女性老年27．532～32．468pg。不同性别MCHC比较，差异有统计学意义（P＜0．05）；参考区间：男328．611～352．810g／L、女323．771～348．750g／L。150例健康体检者中，位于参考区间外者所占百分比均小于10．0％，新建立的参考区间适用于该实验室。结论MCV、MCH和MCHC在成年人群存在性别或（和）年龄差异，按照实际情况分别建立了参考区间，且经验证均适用于该实验室。

Investigation on reference intervals of MCV,MCH and MCHC levels determined by Mindray BC-6800 hematology analyzer

Objective To investigate the reference intervals of mean corpuscular volume (MCV) ,mean corpuscular hemoglo‐bin(MCH) and mean corpuscular hemoglobin concentration (MCHC) examined by the MindrayBC‐6800 hematological analyzer to establish the reference intervals suitable for our laboratory .Methods According to the method recommended by the NCCLS C28‐A3 ,600 healthy adult individuals were selected as the reference individuals .MCV ,MCH and MCHC levels were determined by the MindrayBC‐6800 hematological analyzer for constructing the reference intervals ;other 150 healthy persons undergoing the physical examination were selected and their MCH ,MCV and MCHC detection results were collected for verifying the established reference intervals .Results The detection results of MCV ,MCH and MCHC in healthy adults showed a normal distribution ,MCV had sta‐tistical difference among different age periods (P<0 .05);the reference intervals :82 .278 -94 .242 fL for young adults ,83 .032-94 .608 fL for the middle‐aged persons and 83 .137-96 .343 fL for the elderly .MCH had statistical differences between different se‐xes and among different age periods ;the reference intervals :27 .785-32 .415 pg for male young adults ,28 .324-32 .456 pg for male middle‐aged persons and 28 .274-32 .966 pg for male elderly ;27 .367-31 .973 pg for female young adults ,27 .445-32 .215 pg for female middle‐aged persons and 27 .532 -32 .468 pg for female elderly .MCHC had statistical difference between different sexes (P<0 .05) ;the reference intervals :328 .611-352 .810 g/L for male and 323 .771-348 .750 g/L for female .In 150 individuals un‐dergoing the physical examination ,the proportion of individuals locating at the outside of reference interval was less than 10 .0% , therefore the newly established intervals were suitable for this laboratory .Conclusion The sex difference or/and age differences of MCV ,MCH and MCHC exist among adult populations .So the reference intervals are respectively established according to the prac‐tical situation ,which are suitable for this laboratory by verification .