肿瘤坏死因子-α对少突胶质前体细胞的影响及其机制的研究

目的：探讨肿瘤坏死因子‐α（T N F‐α）对少突胶质前体细胞（O PC ）的作用及机制，为脑室周围白质软化（PV L ）的治疗提供策略。方法将分离纯化的OPC分为空白对照组、TNF‐α组、肿瘤坏死因子受体1（TNFR1）抗体组，将50 ng／mL TNF‐α作用于TNF‐α组、TNFR1抗体组，免疫荧光化学检测OPC的分化情况，四甲基偶氮唑盐（MTT）法检测3组细胞的相对活力， RT‐PCR检测细胞TNFR1的mRNA水平的表达情况。结果与空白对照组和TNFR1抗体组相比，TNF‐α组OPC的细胞活力明显下降（P＜0．05），并且不能沿着少突胶质谱系细胞进行分化，即分化为原少突胶质细胞。TNF‐α组TNFR1的mRNA表达明显的上调，空白对照组和TNFR1抗体组的mRNA表达无明显的变化。结论 TNF‐α主要通过 TNFR1引起OPC的凋亡，抑制O PC分化。

The effect of TNF-alpha on oligodendrocyte progenitor and its mechanism

Objective To explore the effect of tumor necrosis factor‐α(TNF‐α) on the oligodendrocyte progenitor ,and pro‐vide a strategy for the treatment of Periventricular leukomalacia .Methods The purified oligodendrocyte progenitor were divided in‐to three groups :blank control group ,TNF‐αgroup and anti‐TNFR1 group .TNF‐αgroup and anti‐TNFR1 group were treated with TNF‐α,the differentiation of OPC were detected by immunocytochemical method ,the relative viability of cells in three groups were measured by MTT assay ,the levels of TNFR1 gene was observed with RT‐PCR .Results The relative cell viability of TNF‐αgroup was significantly decreased(P<0 .05) and did not turn into cells called pro‐oligodendrocyte .TNF‐αgroup increased the expression of TNFR1 mRNA ,and there were no differences in the expression of TNFR1 mRNA between the blank control group and anti‐TNFR1 group .Conclusion TNF‐αcould induced OPC apoptosis and inhibited the differentiation of OPC through the TNFR1 .