肺炎支原体感染患儿Th1/Th2细胞免疫类型及SOCS1/3 mRNA的表达

目的:观察肺炎支原体(MP)感染患儿早期血清中IFN-γI、L-4的含量和单个核细胞(MNC)中SOCS1、SOCS3的表达,以探讨MP感染的免疫学机制。方法:选取MP感染患儿30例,留取其入院时血样,以健康体检儿童为对照。RT-PCR法测血样MNC中SOCS1,SOCS3的表达,采用ELISA法检测血清中IFN-γ,IL-4的表达水平,并分析其相互关系。结果:MP感染患儿早期IFN-γ的表达和IFN-γ/IL-4的比值比对照组明显增高(P<0.01),IL-4亦稍高(P<0.05);SOCS1的表达较正常对照高(P<0.01),SOCS3的表达较对照无明显差异(P>0.05)。结论:在MP感染早期Th1/Th2均参与了患儿机体的免疫应答,以Th1细胞为主;SOCS1、SOCS3参与了Th1/Th2细胞免疫调节。

Expression of Suppressors of Cytokine Signaling in Mononuclear Cells and the Style of Immunocyte(Th1/Th2) in Children with Mycoplasma Pneumoniae Infection

Objective: To observe the expression of IFN-γ,IL-4 and SOCS1/3 mRNA in serum of children with Mycoplasma pneumoniae(MP) infection,and to discuss the immune mechanism of MP infection.Methods: The expression of IFN-γ and IL-4 in serum of thirty children with MP infection and in fifteen healthy children were detected by ELISA technique.SOCS1/3 mRNA levels in blood mononuclear cells were analyzed by RT-PCR technique.Results: The levels of IFN-γ and IL-4 of MP infected children was obvious higher than those of healthy ones(P<0.01 and P<0.05,respectively);the expression of SOCS1 of MP infected children were higher than that of healthy ones(P<0.01),while the SOCS3 expression was not different between the two groups(P>0.05).Conclusion: Both Th1 and Th2 type of immunoresponse are involved in the course of MP infection,Th1 is primary at the early stage,while SOCS1 and SOCS3 might participate the regulation of Th1/Th2.