非小细胞肺癌中血管内皮生长因子C表达与淋巴结转移关系的研究

目的 探讨血管内皮生长因子C(VEGF-C)与非小细胞肺癌(NSCLC)淋巴管生成和淋巴结转移的关系.方法 52例NSCLC患者分为淋巴结转移阳性组(25例)和淋巴结转移阴性组(27例),采用半定量反转录PCR及免疫组织化学方法 检测2组患者手术切除癌组织及196枚淋巴结组织(2组各98枚,淋巴结转移阳性组98枚中病理阳性淋巴结72枚,阴性26枚)中VEGF-C mRNA及蛋白的表达.结果 淋巴结转移阳性组患者癌组织VEGF-C mRNA表达水平(0.273±0.179)明显高于淋巴结转移阴性组(0.089±0.087,P<0.01);阳性淋巴结组织VEGF.C mRNA表达水平(0.207±0.174)明显高于淋巴结转移阴性组的淋巴结组织(0.114±0.107,P<0.01).在淋巴结转移阳性组中,阳性与阴性淋巴结组织VEGF-C mRNA表达水平分别为0.207±0.174、0.196±0.186,差异无统计学意义(P>0.05).淋巴结转移阳性组15例患者中14例(93.3%)肺癌组织VEGF-C蛋白表达阳性,46枚阳性淋巴结组织中37枚(80.4%)VEGF-c蛋白表达阳性;而淋巴结转移阴性组15例患者中仅1例(6.7%)肺癌组织VEGF-C蛋白表达阳性,52枚淋巴结组织VEGF-C蛋白表达均为阴性,组间差异均有统计学意义(均P<0.01).结论 VEGF-C mRNA与蛋白的表达水平与NSCLC淋巴结转移关系密切,在淋巴结转移的早期诊断中有潜在的应用价值.

Relationship between vascular endothelial growth factor C expression level and lymph node metastasis in non small cell lung cancer

Objective To investigate the relationship between vascular endothelial growth factor C (VEGF-C) expression level and lymph node metastasis in non small cell lung cancer (NSCLC). Methods Fifty-two NSCLC patients, 38 males and 14 females, aged (59±11) (29-77), were divided into 2 groups based on the pathological examination: lymph node metastasis positive group (n=25) and lymph node metastasis negative group (n=27). RT-PCR and immtmohistochemistry were used to detect the mRNA and protein expression of VEGF-C in the tumor tissues and lymph nodes resectod during operation. Reeults The VEGF-C mRNA expression level in the lung tumor tissue of the lymph node metastasis positive group was 0.273±0.179, significantly higher than that in the lymph node metastasis negative group (0.089± 0.087, P<0.01). The VEGF-C mRNA expression level in the positive lymph node of the lymph node metastasis positive group was 0.207±0.174, significantly higher than that in the lymph node metastasis negative group (0.114±0.107, P<0.01), but not significantly different from that in the negative lymph nodes of the same group(0.196±0.186, P>0.05). The VEGF-C protein positive rate of the lung cancer tissues of the lymph node metastasis positive group was 93.3% ( 14/15 ), significantly higher than that of the lymph node metastasis negative group (6.7%, 1/15, P<0.01). The VEGF-C protein positive rate of the metastasis positive lymph noses was 80.4% (37/46), and all 52 metastasis negative lymph nodes were VEGF-C protein negative. Conclusion VEGF-C mBNA and protein expression levels predict lymph node metastasis and can be useful predictors of lymph node metastasis in NSCLC.