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| 组织芯片检测技术在遗传性非息肉性结直肠癌家系筛选中的价值 | |
| 引用本文： | 金黑鹰,丁义江,耿建祥,刘飞,杨柏林,黄书亮,丁曙晴,葛永胜. 组织芯片检测技术在遗传性非息肉性结直肠癌家系筛选中的价值[J]. 中华胃肠外科杂志, 2007, 10(1): 67-69 |
| 作者姓名： | 金黑鹰 丁义江 耿建祥 刘飞 杨柏林 黄书亮 丁曙晴 葛永胜 |
| 作者单位： | 1. 210001,南京中医药大学中西医结合专业博士后流动站 2. 南京中医药大学第三附属医院病理科 3. 南京中医药大学第三附属医院,全国肛肠医疗中心 |
| 基金项目： | 国家自然科学基金资助课题(30572447)，南京市医学发展重点项目资助课题(ZKX05028)，南京市科技人才培养项目(200507037) |
| 摘 要： | 目的研究组织芯片在遗传性非息肉性结直肠癌(HNPCC)家系筛选中的价值。方法将22例HNPCC家系中结直肠癌患者和15例散发性结直肠癌患者的肿瘤组织蜡块制作组织芯片,免疫组化方法检测hMLH1、hMSH2蛋白表达。结果常规免疫组化检测HNPCC组hMLH1或hMSH2阴性68.2%(15/22);散发性结直肠癌组hMLH1阳性14例、阴性1例(6.7%),hMSH2全部阳性。组织芯片检测HNPCC组hMLH1或hMSH2阴性77.2%(17/22);散发性结直肠癌组hMLH1阳性13例、阴性2例(13.3%);hMSH2全部阳性。应用常规免疫组化和组织芯片方法检测hMSH2其结果一致。对hMLH1常规免疫组化和组织芯片技术检测进行比较,P>0.05;差异均无统计学意义。结论组织芯片检测技术是一种高通量的筛选方法,适合于回顾性HNPCC家系筛选。 |
| 关 键 词： | 结直肠肿瘤，遗传性非息肉性 组织芯片 免疫组织化学 家系筛选 |
| 收稿时间： | 2006-06-10 |
Study the value of screening hereditary nonpolyposis colorectal cancer kindreds by detecting the expression of hMLH1/hMSH2 with tissue microarray |
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| JIN Hei-ying,DING Yi-jiang,GENG Jian-xiang,LIU Fei,YANG Bo-lin,HUANG Shu-liang,DING Shu-qin,GE Yong-sheng. Study the value of screening hereditary nonpolyposis colorectal cancer kindreds by detecting the expression of hMLH1/hMSH2 with tissue microarray[J]. Chinese journal of gastrointestinal surgery, 2007, 10(1): 67-69 | |
| Authors: | JIN Hei-ying DING Yi-jiang GENG Jian-xiang LIU Fei YANG Bo-lin HUANG Shu-liang DING Shu-qin GE Yong-sheng |
| Affiliation: | National Center of Colorectal Surgery, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210001, China. jinheiying@yahoo.com.cn |
| Abstract: | Objective To study the value of screening hereditary nonpolyposis colorectal cancer (HNPCC) kindreds by detecting the expressions of hMLH1/hMSH2 with tissue microarray. Methods A tissue microarray with 22 colorectal cancers from HNPCC families and 15 sporadic co]orectal cancers was established,and the expressions of hMLH1/hMSH2 were detected by immunohistochemistry (IHC).Results The expressions of hMLH1 or hMSH2 were negative in 15 of 22 HNPCC and 1 of 15 sporadic colorectal cancers in routine IHC.The expressions of hMLH1 or hMSH2 were negative in 17 of 22 HNPCC and 2 of 15 sporadic colorectal cancers in tissue microarray. The examination of hMSH2 expression yielded same results between routine IHC and tissue microarray. There were no difference on the hMLH1 expressions between routine IHC and tissue microarray. Conclusion Tissue microarray is a high-throughput way to detect the expressions of hMLH1/hMSH2 and is applicable to screen HNPCC kindreds. |
| Keywords: | Colorectal neoplasmas hereditary nonpolyposis Tissue microarray Immunohistochemistry Genealogy screening |
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