| survivin mRNA表达水平与高三尖杉酯碱诱导恶性血液病细胞凋亡的相关性研究 |
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| 引用本文： | 包悍英,蔡真. survivin mRNA表达水平与高三尖杉酯碱诱导恶性血液病细胞凋亡的相关性研究[J]. 浙江大学学报(医学版), 2006, 35(2): 204-208 |
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| 作者姓名： | 包悍英 蔡真 |
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| 作者单位： | 浙江大学医学院附属第一医院,浙江,杭州,310003 |
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| 基金项目： | 教育部留学回国人员科研启动基金;浙江省卫生厅资助项目;浙江省自然科学基金 |
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| 摘 要： | 目的：研究survivin mRNA在细胞株MUTZ-1、K562、Jurkat、RPMI、HL60的表达水平与高三尖杉酯碱（HHT）诱导的凋亡率的关系。方法：应用流式细胞仪技术，研究HHT诱导人骨髓增生异常综合征细胞株MUTZ-1、人急性红白血病细胞株K562、人淋巴瘤细胞株Jurkat和人骨髓瘤细胞株RPMI，以及人急性粒细胞白血病细胞株HL60细胞凋亡。用半定量逆转录酶-多聚酶链式反应，检测上述细胞株及HHT作用MUTZ-1细胞后凋亡蛋白抑制因子survivin、XIAP的表达。结呆：细胞株MUTZ-1、K562、Jurkat、RPMI、HL60均表迭survivin、XIAP mRNA，HHT能诱导各细胞株凋亡，HHT诱导的凋亡率与细胞株survivin mRNA表达呈负相关。结论：survivin在细胞中的表达水平可能直接影响细胞对化疗药物的敏感性，可作为预测细胞耐药的指标之一，同时也可能将成为肿瘤治疗的新靶向。
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| 关 键 词： | 血液病 三尖杉酯碱类/药理学 细胞株 细胞凋亡 高三尖杉酯碱 |
| 文章编号： | 1008-9292（2006）02-0204-05 |
| 收稿时间： | 2004-10-17 |
| 修稿时间： | 2005-02-25 |
Expression of survivin mRNA in HHT-induced cell apoptosis of hematological malignancy cell lines |
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| BAO Han-ying,CAI Zhen. Expression of survivin mRNA in HHT-induced cell apoptosis of hematological malignancy cell lines[J]. Journal of Zhejiang University. Medical sciences, 2006, 35(2): 204-208 |
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| Authors: | BAO Han-ying CAI Zhen |
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| Affiliation: | The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. |
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| Abstract: | Objective: To investigate the expression of survivin mRNA in hematological malignancy cells and its correlation with HHTinduced cell apoptosis.Methods: Hematological malignancy cell lines MUTZ-1,K562,Jurkat,RPMI and HL60 were treated with HHT in vitro.Cell apoptosis was observed by flow cytometry(FCM) and the expression of surviving and XIAP mRNA was evaluated with semi-quantitative RT-PCR.Results: The expression of survivin mRNA on cell lines was negatively correlated to HHT-induced cell apoptotic rate(r=-0.980,P=0.003).There were no significant differences in XIAP expression among these 5 cell lines.Conclusion: Survivin could be used as a new marker for drug-sensitivity and a new target for treatment of hematological malignancies. |
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| Keywords: | MUTZ-1 survivin XIAP |
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