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| 应用蛋白质指纹图谱技术进行肝纤维化早期诊断的实验研究 | |
| 引用本文： | 董永杰,彭彦辉,赵晓云.应用蛋白质指纹图谱技术进行肝纤维化早期诊断的实验研究[J].中国普外基础与临床杂志,2010,17(5):459-462. |
| 作者姓名： | 董永杰 彭彦辉 赵晓云 |
| 作者单位： | 1. 邯郸市中心医院普外科,河北邯郸,056001 2. 河北省人民医院普外科,石家庄,050051 3. 河北省老年医学实验室,石家庄,050051 |
| 摘 要： | 目的检测肝纤维化各期的血清蛋白质组图谱,筛选特异的蛋白质标志物,用于肝纤维化的早期诊断。方法 SD大鼠50只,随机分为对照组(n=6)和实验组(n=44)2组,采用表面增强激光电离飞行时间质谱技术检测2组大鼠血清中的差异蛋白;采用放射免疫法检测血清肝纤维化4项,即Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)、层粘连蛋白(LN)和透明质酸酶(HA)。结果实验组中属肝纤维化Ⅰ、Ⅱ、Ⅲ及Ⅳ期者分别有8、5、5及6只〔有20只大鼠未到实验设定的采集标本时间死亡(系注射CCl4所致)〕。发现了质核比为4 203、4 658及7 400的3个差异蛋白标志物,其中质核比为4 658及7 400的2个蛋白质峰在肝纤维化Ⅰ期即明显升高,与对照组比较差异有统计学意义(P0.05);而血清肝纤维化4项的水平在肝纤维化Ⅲ期或Ⅳ期才明显高于对照组(P0.05)。结论蛋白质指纹图谱技术在肝纤维化的早期诊断以及标志物筛选方面有一定的价值,值得进一步深入研究。 |
| 关 键 词： | 肝纤维化 差异蛋白 蛋白质指纹图谱技术 蛋白质组学 蛋白质芯片 |
Experimental Study of Serum Proteomics Patterns Detected by SELDI-TOF MS in Early Diagnosis of Hepatic Fibrosis |
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| DONG Yong-jie,PENG Yan-hui,ZHAO Xiao-yun.Experimental Study of Serum Proteomics Patterns Detected by SELDI-TOF MS in Early Diagnosis of Hepatic Fibrosis[J].Chinese Journal of Bases and Clinics In General Surgery,2010,17(5):459-462. | |
| Authors: | DONG Yong-jie PENG Yan-hui ZHAO Xiao-yun |
| Institution: | DONG Yong-jie1,PENG Yan-hui2,ZHAO Xiao-yun3.1.Department of General Surgery,The Central Hospital of H,an,H,an 056001,Hebei Province,China,2.Department of General Surgery,The People\'s Hospital of Hebei Province,Shijiazhuang 050051,3.Laboratory of Geriatric Medicine of Hebei Province |
| Abstract: | Objective To find a new specific marker that can be used to early diagnose hepatic fibrosis by detecting the change of serum protein in patients with hepatic fibrosis.Methods This research adopted 50 SD rats(25 males and 25 females),and from which 6 rats were selected randomly(3 males and 3 females) as control group,last 44 rates were divided into four groups according to four pathological stages as hepatic fibrosis model group(experimental group).Distinct proteins in serum were detected by surface-enhanced... |
| Keywords: | Hepatic fibrosis Distinct protein Protein finger printing technology Proteomics Protein chip |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |