小鼠骨髓细胞肾上腺素β2受体的表达及其意义

目的：探讨骨髓细胞肾上腺素β₂受体(β₂-AR)的表达及其意义，为神经因素调节骨髓造血理论提供依据。方法：用Ficoll淋巴细胞分离液从正常小鼠骨髓分离的单个核细胞直接涂片、粒-单集落造血祖细胞培养后涂片、骨髓基质细胞培养爬片，同时行骨髓石蜡切片及全骨髓细胞涂片，用免疫组织化学SP法检测上述标本中骨髓细胞β₂-AR的表达。另取小鼠采用淋巴细胞注射法制备免疫介导的再生障碍性贫血（简称再障）模型，β₂-AR拮抗剂组静脉注射ICI 118551，对照组静脉注射生理盐水，至第8天采用Western blotting 检测3组小鼠骨髓单个核细胞β₂-AR的表达。结果：正常小鼠骨髓细胞β₂-AR免疫组织化学反应可见胞质及胞膜呈深浅不等的棕黄色，其中单个核细胞48%强阳性，造血祖细胞55%中等阳性、45%弱阳性，骨髓基质细胞68%强阳性，其他辅助细胞呈不同程度的阳性反应。再障模型组、β₂-AR拮抗剂组及对照组小鼠骨髓单个核细胞β₂-AR相对表达量分别为1.03±0.31、1.72±0.29及1.41±0.35。与对照组比较，再障模型组β₂-AR表达量明显降低（P<0.05），β₂-AR拮抗剂组β₂-AR表达明显增加（P<0.05）。结论：正常小鼠骨髓细胞有β₂-AR表达，其表达量与骨髓造血状态有关，提示β₂-AR可能通过神经-内分泌-免疫网络途径参与造血调节。

Expression of beta 2 adrenergic receptor in marrow cells of mice and its significance

Objective To explore the expression of beta 2 adrenergic receptors(β2-AR) in bone marrow cells and its significance and provide a scientific basis for the theory of hematopoietic regulation by nerve factors.Methods After bone marrow mononuclear cells were isolated from normal mouse bone marrow by the Ficoll density gradient centrifugation,the expressions of β2-AR were studied by immunohistochemistry in bone marrow mononuclear cells,cultured bone marrow stromal cells,GM-CFUs,paraffin sections and smears of bone marrow.Mouse models of immune-mediated aplastic anemia(AA) were made by lymphocyte infusion.Mice were divided into 3 groups including animal model group,β2-AR antagonist (ICI 118551) group,and control group,and the expression of β2-AR in bone marrow mononuclear cells at the 8 th day was detected by Western blotting.Results The positive expression of β2-AR in cytoplasm or membrane of normal mouse bone marrow cells showed different brown by immunohistochemical staining.There were intensive positive expressions of β2-AR in mononuclear cells(48%) and bone marrow stromal cells (68%),while progenitor cells only showed middle-positive(55%)or weak positive(45%),and other hematopoietic accessory cells were positive in varying degrees.The relative values of β2-AR of bone marrow mononuclear cells in 3 groups were 1.03±0.31,1.72±0.29 and 1.41±0.35,respectively;the expression of β2-AR in animal model group was more lower than that in control group(P<0.05),and the expression of β2-AR in β2-AR antagonist group was more higher than that in control group(P<0.05).Conclusion β2-AR can express in bone marrow cells of normal mice, and the expression level of β2-AR is related to hematopoietic function of bone marrow,it may be involved in hematopoietic regulation by neuro-endocrine-immunity net work.