| 胆固醇对血管内皮细胞基因组DNA甲基化的影响 |
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| 引用本文： | 卢志顺,余晓,唐俊利,钱民章. 胆固醇对血管内皮细胞基因组DNA甲基化的影响[J]. 江苏大学学报(医学版), 2007, 17(1): 61-62 |
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| 作者姓名： | 卢志顺 余晓 唐俊利 钱民章 |
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| 作者单位： | 遵义医学院生物化学教研室,贵州,遵义,563003;遵义医学院生物化学教研室,贵州,遵义,563003;遵义医学院生物化学教研室,贵州,遵义,563003;遵义医学院生物化学教研室,贵州,遵义,563003 |
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| 摘 要： | 目的:研究胆固醇对人血管内皮细胞基因组DNA甲基化水平的影响.方法:体外培养人脐静脉血管内皮细胞ECV304,以50.00 mg/L的胆固醇作用于ECV304 48 h,提取细胞基因组DNA,用高效液相色谱法(HPLC)检测基因组DNA甲基化水平.结果:与无胆固醇的对照组比较,50.00 mg/L胆固醇使ECV304细胞中DNA甲基化水平显著下降(P＜0.01).结论:一定浓度的胆固醇能降低血管内皮细胞中DNA甲基化水平.
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| 关 键 词： | 胆固醇 血管内皮细胞 基因组DNA 甲基化 |
| 文章编号： | 1671-7783（2007）01-0061-02 |
| 收稿时间： | 2006-09-19 |
| 修稿时间： | 2006-09-19 |
Effect of cholesterol on genomic DNA methylation level of human vascular endothelial cells |
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| LU Zhi-shun,YU Xiao,TANG Jun-li,QIAN Min-zhang. Effect of cholesterol on genomic DNA methylation level of human vascular endothelial cells[J]. Journal of Jiangsu University Medicine Edition, 2007, 17(1): 61-62 |
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| Authors: | LU Zhi-shun YU Xiao TANG Jun-li QIAN Min-zhang |
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| Affiliation: | Department of Biochemistry, Zunyi Medical College, Zunyi Guizhou 563003 ,China |
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| Abstract: | Objective: To investigate the influence on the methylation level of genomic DNA caused by cholesterol on the vascular endothelial cells(VECs).Methods: Cholesterol(50.00 mg/L) was used on human umbilical endothelial cell line,ECV304,48 h later,cell genomic DNA methylation level was detected by HPLC. Results: Compared with the untreated control group,the genomic DNA methylation level significantly decreased when cell was treated by 50.00 mg/L cholesterol. Conclusion: The specifical concentration of cholesterol could decrease the VECs genomic DNA methylation level. |
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| Keywords: | cholesterol vascular endothelium cell genomic DNA methylation |
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