致敏的树突状细胞治疗对黑色素瘤小鼠体内TNF-α及IL-6水平的影响

目的研究致敏的树突状细胞(DC)治疗后黑色素瘤(B16)小鼠体内TNF-α及IL-6的变化规律.方法用冻融抗原致敏BALB/C小鼠骨髓来源的DC.ELISA法检测治疗组荷瘤小鼠、荷瘤未治组小鼠及正常小鼠体内TNF-α及IL-6水平.结果随时间荷瘤小鼠体内IL-6水平逐渐升高,经治疗后IL-6水平升高更为显著,而荷瘤未治组小鼠TNF-α水平逐渐降低经治疗后TNF-α水平显著升高.结论经过抗原致敏的DC治疗,荷瘤鼠体内TNF-α、IL-6水平显著升高,表明抗原致敏的DC能够增强荷瘤小鼠抗肿瘤免疫反应.

Level of TNF - α and IL - 6 in melanoma - bearing mice after treatment by dendritic cells pulsed with tumor cell lysates

Objective: To investigate the mechanism in melanoma - bearing mice after treatment by dendritic cells′pulsed with tumor cell lysates. Methods: Dendritic cells generated from murine bone marrow were pulsed with B16 tumor lysates 40 BALB/C mice were divided into 3 groups: normal group without therapy, bearing tumor group without therapy, bearing tumor group with therapy. The level of TNF - α and IL - 6 was detected in 3 groups by ELISA. Results: The level of the serum IL - 6 of bearing tumor mice was higher than that of control group mice (P＜ 0. 01 ), furthermore the level of the IL - 6 of the mice treated with tumor tysate - pulsed DCs was the highest (P ＜0.01 ) in three groups. The level of TNF - α of bearing tumor group was lower than that of control group(P ＜ 0.0 1 ),and the TNF -α level of treated group was obviously higher than that of control group and beating tumor group(P ＜0.01 ). The level of IL - 6 and TNF - α bearing tumor mice in vivo increased obviously after therapy of tumor lysate -pulsed DCs. Conclusions: Tumor lysate - pulsed DCs enhance anti - tumor immunity effect.