小鼠肾包膜下接种法（SRCA）实验研究的病理组织学观察

观察了96例不同组织类型实体瘤,经重量法评价系统评价,78例R值≥1,可评价率为81.3％;无可评价比18/96(R值<1)。光镜观察发现,部分接种瘤块瘤细胞保存不理想;进一步的研究结果表明,未经药物处理的植入瘤块重量变化与病理组织学观察中的瘤细胞保存欠佳的分析结果很不一致,统计学显示高度差异性;接种癌块靠近肾实质一侧有数量不等的宿主炎细胞浸润,但程度较轻,经相关统计分析,宿主炎细胞浸润与植入瘤块的重量变化无相关关系(P>0.05)。被接种瘤块取材是否均质,实验结果的影响显著不同。文中对不同肿瘤类型在以上实验基础上与药物作用的反应进行了分析讨论。

THE EXPERIMENTAL STUDY ON THE HISTOPATHOLOGICAL CHANGES IN THE SUBRENAL CAPSULE ASSAY

The histopathological changes in the experimental study in 4-5 dayssubrenal capsule assay were reported in 96 cases of various types of solid tumors. Under the observations by light microscope, the tumor cells in some of the implants were preserved poorly. The results of further experiments showed that the change of the size of untreated grafts was not correlated to the pathological findings of the tumor with poorly preserved tumor cells, and the difference highly significant. It was found that homogeneity of the implanted tumor tissue could appreciably affect the experimental results. There were some host cell infiltration in the graft near the side adhering to the kidney parenchyma, but statistics, showed that there was no correlation between the change of the size of the graft and the extent of the host cell infiltration (P>0.05). We considered that the edema of grafts in various degrees was one of the important factors affecting on the increase in weight and dimensions. The reaction of anticancer drugs to various types of solid tumors in this experimental system was also discussed in this article.