非小细胞肺癌中survivin 基因表达及其与p53 、bcl-2 基因表达的相互关系

 目的 探讨survivin基因在非小细胞肺癌（NSCLC）中的表达，及其与p53、bcl-2蛋白表达的相互关系。方法 应用免疫组织化学链霉茵抗生物素蛋白-过氧化酶连接法（SP法），检测survivin、p53、bcl-2基因在76例NSCLC肿瘤组织及survivin基因在30例病灶旁正常肺组织中的表达。结果 survivin基因在正常肺组织中不表达，76例肺癌组织中46例表达阳性，占60．5％。survivin基因表达与肺癌患者的年龄、性别、吸烟、病理类型、分化程度、肿瘤大小、淋巴结转移及TNM分期无明显相关关系。肺癌组织中p53蛋白表达阳性、阴性者中，survivin基因表达阳性率分别为71．7％（33／46）、43．3％（13／30），两者比较，差异有显著性（P〈0．05）；bcl-2蛋白表达阳性、阴性者中，survivin基因表达阳性率分别为94．7％（36／38）、26．3％（10／38），两者比较，差异有极显著性（P〈0．01）。结论 survivin基因在肺癌组织中的异常表达，对NSCLC的发生发展起重要作用。survivin基因表达与肺癌组织中p53、bcl-2蛋白的异常表达密切相关。survivin基因可能成为NSCLC新的诊断标志及基因治疗的新靶点。

Expression of survivin Gene and Its Relationship with Expression of p53, bcl-2 Genes in Non-small-cell Lung Cancer

Objective To study the expression of a novel inhibitor gene of apoptosis, survivin, in non-small-cell lung cancer and its relationship with the expression of p53, bcl-2 genes. Methods Using streptavidin-biotin peroxidase (SP) method, we examined the expression of survivin, p53 and bcl-2 genes in 76 NSCLC tumor samples and the expression of survivin gene in 30 adjacent normal lung tissue samples. Results survivin was expressed in 46 of 76 ( 60.5%) cases of lung cancer but was not expressed in 30 cases of adjacent normal lung tissues. There was no relationship between survivin gene expression and age, sex, cigarette smoking, histologic subtyre, differentiation, tumor size, lymph node metastases, or TNM Stages. survivin positive cases were strongly associated with p53 expression (33/46, 71.7% versus 13/30 43.3%, P< 0.05) and bcl-2 expression (36/38, 94.7% versus 10/38 26.3%, P< 0.01). Conclusions The survivin gene was expression in a vast majority of NSCLC, it also has a great relationship with the expression of p53 and bcl-2. survivin gene may play a role in the pathway of carcinogenesis and be identified as a defining diagnostic marker and a potential therapeutic target in NSCLC.