基于酶抑制原理的电化学生物传感器在药物检测中的研究进展

目前多种疾病的病理机制都与酶息息相关，酶抑制剂类药物的开发也呈指数增长。构建复合酶电极的电化学生物传感器能够对酶抑制剂类药物进行简单、快速的分析检测。此类传感器根据电极材料的不同特性选择合适的单一或复合材料固定化酶，制备生物识别受体元件，用以检测待测物的酶抑制活性。按照与人体疾病和药物机制联系较为紧密的几类靶标酶为分类，综述了近5年生物传感器在酶抑制剂类药物检测中的应用进展，为后续筛选作为酶抑制剂的小分子先导化合物的进一步研究提供理论依据。

Recent Advances in Research on Electrochemical Biosensors based on Enzyme Inhibition for Drug Detection

The pathogenesis of many diseases is closely related to enzymes, and the development of enzyme inhibitors is also increasing exponentially. The electrochemical biosensor constructed with composite enzyme electrode can analyze and detect enzyme inhibitor drugs effectively and efficiently. According to the various characteristics of the electrode materials, single or composite materials are selected for immobilizing the enzymes, and then a biorecognition receptor is formed to detect the enzyme inhibition activity of the analyte. In this review, we summarized the advances of the biosensors in the detection of enzyme inhibitor drugs in the past five years in terms of target enzymes that are closely related to human diseases and drug mechanisms, which provides a theoretical basis for further screening of small molecule lead compounds as enzyme inhibitors.