冷冻冷藏食品小肠结肠炎耶尔森菌病原学检测

  目的  通过对宁夏地区小肠结肠炎耶尔森菌的宿主分布及毒力基因情况进行分析，能够有效防止该菌蔓延扩散。  方法  对2012 — 2017年不同监测样本进行菌株分离培养，并对阳性菌株做血清凝集和毒力基因鉴别。  结果  2012 — 2017年，共采集各类样本8 032份，分离小肠结肠炎耶尔森菌146株，总检出率为1.81 %。同时携带ail，ystA，yadA，virF毒力基因的致病性菌株全部来自O : 3血清型，均来源于动物样本。  结论  宁夏地区广泛存在小肠结肠炎耶尔森菌，其中猪的致病性带菌率最高，且具有高度同源性，不存在时间差异。

Distribution of pathogenic Yersinia enterocolitica in China

  Objective  To examine the distribution of host and virulence gene of Yersinia enterocolitis in Ningxia Hui Autonomous Region（Ningxia）for effective control of the spread of the bacteria.  Methods  We collected various kinds of specimens in Ningxia from 2012 to 2017 for the culture and isolation of Yersinia enterocolitis strains; then serum agglutination test and virulence gene identification were performed for the isolated strains.  Results  A total of 8 032 specimens were collected during the 6-year period and 146 Yersinia enterocolitis strains were indentified, with the detection rate of 1.81%. The pathogenic strains carrying ail, ystA, yadA, virF virulence gene were all belong to O : 3 serotype and were isolated from animal specimens.  Conclusion  Yersinia enterocolitica is wide-spread in Ningxia. The detection rate of pathogenic Yersinia enterocolitica strains is the highest for specimens collected from pigs and the isolated strains are of high homology and of no difference in temporal distribution.