胃癌癌前病变中医证候与凋亡相关癌基因mRNA表达的关系

【目的】 探讨胃癌癌前病变(PLGC)中医证候与bcl-2癌基因和p53抑癌基因mRNA表达的关系。【方法】经胃镜及病理证实为PLGC病例共40例,其中中度异型增生24例,重度异型增生9例,不完全性结肠化生7例;兼气滞证10例,兼胃热证12例,兼血瘀证18例。采用原位分子杂交的方法检测胃粘膜活检标本bcl-2癌基因和p53抑癌基因mRNA表达。【结果】本组PLGC中,bcl-2癌基因和p53抑癌基因在转录水平均有过度表达,并随病变的进展而表达逐渐增高。在不同的兼证中,bcl-2癌基因和p53抑癌基因mRNA的表达强度依次为兼气滞证<兼胃热证<兼血瘀证。【结论】bcl-2癌基因和p53抑癌基因在PLGC中有异常表达,其表达与不同兼证有关且可能有一定的证候特异性。

Relationship Between Syndrome Patterns of Traditional Chinese Medicineand Apoptosis- Related Oncogene mRNA Expressionsin Precancerous Lesions of Gastric Cancer

Objective] To study the relationship between syndrome patterns of traditional Chinese medicine and bcl- 2 oncogene, p53 suppression gene mRNA expressions in precancerous lesions of gastric cancer (PIGC). Methods] Forty PLGC cases were endoscopically and pathologically confinned, including 24 cases of moderate dysplasia of gastric mucosa, 9 cases of severe dysplasia, 7 cases of incomplete colonic intestinal metaplasia. Of the 40 cases, 10 were complicated with Qi stagnation, 12 with stomach-heat, and 18 with blood stasis, mRNA expression of bcl- 2 oncogene and p53 suppression gene were detected in situ by molecular hybridization method. Results] The mRNA overexpression of bcl- 2 oncogene and p53 suppression gene were found in PLGC, and the expression was gradually increased with the progress of lesions. In the complicated cases, the mRNA expression of bcl- 2 oncogene and p53 suppression gene were the least in the cases with Qi stagnation and less in the cases with stomach- heat than in the cases with blood stasis. Conclusion] Abnormal mRNA expression of bcl- 2 oncogene and p53 suppression gene were found in PLGC cases and related with different complicated cases, indicating the specificity of different syndrome patterns.