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| 肢体缺血/再灌注诱发大鼠心脏血红素氧合酶1的表达(英文) | |
| 引用本文： | 丁延峰,李东亮,周君琳,金国华,刘虹彬,薛保建,沈鸿宾. 肢体缺血/再灌注诱发大鼠心脏血红素氧合酶1的表达(英文)[J]. 新乡医学院学报, 2001, 18(2): 77-81 |
| 作者姓名： | 丁延峰 李东亮 周君琳 金国华 刘虹彬 薛保建 沈鸿宾 |
| 作者单位： | 1. 新乡医学院生理教研室, 2. 河北医科大学第三附属医院 3. 生化教研室 4. 河北医科大学生理研究室 5. 河北科技出版社 |
| 基金项目： | Supported by NationalNatural Science Foundation of China (No.30070282) |
| 摘 要： | 目的 观察后肢体缺血 /再灌注是否诱发心脏血红素氧合酶 1(HO - 1)的表达。方法 在氨基甲酸乙脂麻醉大鼠行后肢体缺血 /再灌注后 ,测量心脏组织中丙二醛的含量和超氧歧化酶 (SOD)的活性 ;并应用Northern和Western杂交方法分别检测HO - 1mRNA和蛋白的表达情况。结果 在肢体缺血 /再灌注过程中 ,心脏中的丙二醛含量增加 ,而超氧歧化酶的活性降低 ;单纯肢体缺血并不引起心脏血红素氧合酶 1mR NA和蛋白表达的变化 ,但缺血 /再灌注增强心脏血红素氧合酶 1mRNA和蛋白表达。结论 肢体缺血 /再灌注不仅提高心脏中丙二醛的含量 ,降低超氧歧化酶的活性 ;而且上调血红素氧合酶 1在心脏的表达。 |
| 关 键 词： | 血红素氧合酶 热休克蛋白 基因表达 心脏 肢体缺血/再灌注 大鼠 |
| 文章编号： | 1004-7239(2001)02-0077-05 |
| 修稿时间： | 2000-12-10 |
Limb ischemia/reperfusion induces haem oxygenase 1 expression in rat heart |
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| DING Yan-feng,ZHOU Jun-lin,JIN Guo-hua,LIU Hong-bin,XUE Bao-jian,LI Dong-liang,SHEN Hong-bin. Limb ischemia/reperfusion induces haem oxygenase 1 expression in rat heart[J]. Journal of Xinxiang Medical College, 2001, 18(2): 77-81 | |
| Authors: | DING Yan-feng ZHOU Jun-lin JIN Guo-hua LIU Hong-bin XUE Bao-jian LI Dong-liang SHEN Hong-bin |
| Affiliation: | DING Yan-feng1,ZHOU Jun-lin2,JIN Guo-hua2,LIU Hong-bin3,XUE Bao-jian4,LI Dong-liang1,SHEN Hong-bin5 |
| Abstract: | Objective To observe whether hind limb ischemia/reperfusion induces the haem oxygenase 1 (HO-1) expression in rat heart. Methods The urethane-anesthetized rats were undergone with hind limb ischemia/reperfusion. The contents of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in heart were detected. The expressions of HO-1 mRNA and protein in myocardium were measured by Northern blotting and Western blotting respectively.Results During limb ischemia/reperfusion, the content of MDA was increased and the activity of SOD was decreased in heart. The simple limb ischemia didn't induce the expressions of HO-1 mRNA and protein, but the levels of HO-1 mRNA and protein in the myocardium were increased by limb ischemia/reperfusion. Conclusion Limb ischemia/reperfusion could not only increase the content of MDA and decrease the activity of SOD in heart, but also upregulate the expression of HO-1 in heart. |
| Keywords: | haem oxygenase heat shock protein gene expression heart limb ischemia/reperfusion rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |