HPV31型L2保守中和表位可诱发广谱中和抗体

目的研究人乳头瘤病毒31型(HPV31)次要外壳蛋白L2保守中和表位的免疫活性及诱发抗体的中和范围。方法合成法获得HPV31 L2 aa.17-40多肽,用EDC法偶联KLH,联合弗氏佐剂免疫新西兰大白兔,用假病毒中和实验检测免疫血清对来自α4、α7、α9、α10及β1亚属的多个HPV型别的中和抗体。结果 HPV31 L2-KLH偶联肽可在新西兰大白兔体内诱发针对至少17种HPV型别的广谱中和抗体,其中HPV31的中和抗体滴度最高,HPV5/45/57的次之。结论首次发现HPV31 L2保守中和表位免疫血清具有广谱中和活性,为基于该表位的广谱HPV疫苗研发奠定了基础。

Conservative epitope of human papillomavirus type 31 L2 can induce broad-spectrum neutralizing antibodies

Objective To detect the immunogenicity of conservative neutralizing epitope from human papilloma-virus type 31 ( HPV31) minor capsid protein L2, and analyze the neutralizing antibody spectrum of its immune sera.Methods Synthesize HPV31 L2 aa.17-40 peptide and conjugate with KLH by EDC.Immunize New Zeal-and white rabbits with HPV31 L2-KLH and Freud's Adjuvant.Analyze the neutralizing antibody titers of immune sera against HPVs from α4,α7,α9,α10 and β1 species by pseudovirus neutralization assay.Results In rabbits, HPV31 L2-KLH induced broad-spectrum neutralizing antibodies against at least 17 types of HPV, among which the neutralizing antibody titers against HPV31 are the highest, followed by that of HPV5/45/57. Conclusions The broad-spectrum neutralizing activity of the immune sera of HPV31 L2 is stimulated by conser-vative neutralizing epitope.The results lay the foundation of the development of broad-spectrum HPV vaccines based on HPV31 L2 neutralizing epitope.