白藜芦醇抑制高糖诱导的H9C2心肌细胞系肥大

目的观察白藜芦醇对高糖诱导H9C2心肌细胞系氧化应激和肥大的作用及其可能机制。方法将H9C2心肌细胞分为正常糖浓度组(NG组)、高糖组(HG组)、白芦藜醇组(Res组)和白藜芦醇+DAPT组(Res+DAPT组)。用鬼笔环肽检测细胞表面积;DCFH-DA法检测细胞内活性氧簇(ROS);比色法检测超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;实时荧光定量PCR及Western blot检测Notch1、Hes1、ANP、BNP mRNA和蛋白表达。结果与NG组相比,高糖可诱导心肌细胞肥大(P0.01),ROS和MDA含量增加(P0.01),SOD活性下降(P0.01),伴随ANP和BNP基因及蛋白表达增加(P0.05),Notch1和Hes1表达下降(P0.05),白藜芦醇处理后可逆转心肌细胞肥大(P0.01),减轻ROS和MDA氧化损伤(P0.01),增加SOD活性(P0.01),抑制ANP和BNP表达(P0.05),增加Notch1及Hes1表达(P0.05)。结论白藜芦醇可能通过改善氧化应激,活化Notch1/Hes1信号通路抑制高糖引起的H9C2心肌细胞肥大。

Resveratrol inhibits high glucose-induced hypertrophy in myocardial cell line H9C2

Objective To investigate the effect of resveratrol on high glucose-induced oxidative stress and cardiomyo-cyte hypertrophy and possible mechanism .Methods H9C2 cardiomyocytes were divided into normal glucose group , high glucose group , resveratrol group and resveratrol +DAPT group respectively .The cell surface was measured by rhodamine-labeled phalloidin .Reactive oxygen species generation was measured using DCFH-DA.The contents of SOD and MDA were evaluated by colorimetry .The mRNA and protein levels of Notch 1, Hes1, ANP and BNP were evaluated by RT-qPCR and western blot .Results High glucose exposure significantly increased cell surface area ( P<0.01) and enhanced ROS and MDA generation ( P <0.01 ) with concomitant decrease of SOD activity ( P <0.01).It also decreased the mRNA and protein expressions of Notch 1 and Hes1(P<0.05), and increased the ex-pression levels of ANP and BNP ( P<0.05 ) .Resveratrol treatment inhibited cell surface expanding ( P<0.01 ) and decreased ROS and MDA generation (P<0.01) with concomitant enhance SOD activity (P<0.01), as well as in-creased the expression level of Notch 1 and Hes1 ( P<0.05 ) , and decreased the expression of ANP and BNP ( P<0.05).Conclusions Resveratrol may inhibit high glucose induced H9C2 cardiomyocytes hypertrophy by improving oxidative stress and activating Notch 1/Hes1 signaling pathway .